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Engineering of Chitosan-derived nanoparticles to enhance antimicrobial activity against foodborne pathogen Escherichia coli O157:H7
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2018-06-12 , DOI: 10.1016/j.carbpol.2018.06.046
Alejandro Garrido-Maestu , Zhengxin Ma , Sae-Yeol-Rim Paik , Nusheng Chen , Sanghoon Ko , Zhaohui Tong , KwangCheol Casey Jeong

Chitosan is an abundant and natural polymer derived from chitin, which presents a wide variety of properties, including antimicrobial activity. The raising of antibiotic resistant bacteria has increased the interest in finding alternatives to traditional antibiotics. Many studies have assessed the antimicrobial activity of chitosan itself, but a few have performed comparisons among different chitosan nanoparticle synthesis, which will be of particular interest for further applications. In this study, the effects of two types of cross-linking agents, sodium sulfate vs. tripolyphosphate, along with molecular weight (Mw) of chitosan, low vs. high Mw, and different sonication treatments, time and power, were assessed to determine the optimal conditions to enhance antimicrobial activity against bacterial pathogens. Physiochemical characteristics of the engineered chitosan nanoparticles were determined. It was observed that 20 min sonication time, low Mw of chitosan, Sodium sulfate as cross-linker, and particle size smaller than <300 nm, showed the greatest antimicrobial activity. Chitosan nanoparticles generated at this condition completely killed pathogenic E. coli O157:H7 without raising resistant bacteria, providing great insights into potential use as alternative antimicrobial agents.



中文翻译:

壳聚糖衍生的纳米颗粒的工程化,以增强对食源性病原体大肠杆菌O157:H7的抗菌活性

壳聚糖是衍生自甲壳质的丰富而天然的聚合物,具有多种特性,包括抗微生物活性。抗生素抗性细菌的产生增加了寻找传统抗生素替代品的兴趣。许多研究已经评估了壳聚糖本身的抗菌活性,但是一些研究对不同的壳聚糖纳米颗粒合成方法进行了比较,这对于进一步的应用将特别有意义。在这项研究中,评估了两种交联剂的影响:硫酸钠与三聚磷酸钠,以及壳聚糖的分子量(Mw),低与高Mw以及不同的超声处理,时间和功率,以确定增强对细菌病原体的抗菌活性的最佳条件。确定了工程化壳聚糖纳米颗粒的理化特性。观察到超声处理时间为20分钟,壳聚糖的低Mw,作为交联剂的硫酸钠和小于300 nm的粒径均显示出最大的抗菌活性。在这种条件下产生的壳聚糖纳米颗粒完全杀死了致病菌大肠杆菌O157:H7,不产生抗药性细菌,为潜在的替代抗菌剂用途提供了深刻的见识。

更新日期:2018-06-12
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