In the present study, the electrochemical behavior of the antiviral drug tenofovir (tnf) on a boron-doped diamond electrode (BDDE) has been studied using square-wave voltammetry (SWV). The experimental conditions such as the supporting electrolyte composition and the SWV parameters were optimized. Under the optimized conditions, a simple and sensitive SWV procedure for tnf determination was developed in the concentration range of 5.0 × 10⁻⁶ to 1.0 × 10⁻⁴ mol L⁻¹. The calculated limit of detection and limit of quantification were equal to 5.6 × 10⁻⁷ and 1.9 × 10⁻⁶ mol L⁻¹, respectively. The biological significance of the developed method was demonstrated by a quantitative analysis of the pharmaceutical formulations Viread and Tenofovir disoproxil Teva. Moreover, both voltammetric and spectroscopic techniques were used to study the interaction between tnf and DNA. Changes in the electrochemical and spectroscopic behavior of tnf in the presence of DNA were used to calculate the binding constants of the formed complexes. The estimated values of Gibbs free energy revealed that the formation of the drug–DNA complexes was a spontaneous and favorable process. Moreover, for free and bound tenofovir, kinetic parameters such as heterogeneous rate constant, electron transfer coefficient, and diffusion coefficient were determined.

在本研究中，已使用方波伏安法（SWV）研究了抗病毒药物替诺福韦（tnf）在掺硼金刚石电极（BDDE）上的电化学行为。优化了实验条件，例如支持电解质的组成和SWV参数。在优化的条件下，开发了一种简单而灵敏的tnf测定的SWV程序，其浓度范围为5.0×10 ^-6至1.0×10 ^-4  mol L ^-1。计算出的检出限和定量限分别为5.6×10 ^-7和1.9×10 ^-6  mol L ^-1， 分别。通过对药物制剂Viread和Tenofovir disoproxil Teva进行定量分析，证明了所开发方法的生物学意义。此外，伏安法和光谱法都用于研究tnf和DNA之间的相互作用。在DNA存在下，tnf的电化学和光谱行为的变化用于计算形成的复合物的结合常数。吉布斯自由能的估计值表明，药物-DNA复合物的形成是一个自发的，有利的过程。此外，对于游离和结合的替诺福韦，确定动力学参数，例如异质速率常数，电子转移系数和扩散系数。