The sensitive simultaneous detection of multiple biomarkers is critical for the early diagnosis of diseases. Electrochemiluminescence (ECL) offers outstanding advantages, e.g., low background, over other optical sensing techniques. However, multiplexed ECL bioassay is hindered not only by the lack of generally available ECL spectrometers but also by the limited number of biocompatible monochromatic ECL luminophores for decades. Herein, we report addressing these issues by re-examination of the recent tabletop spectrofluorometer coupled potentiostat as a high-resolution ECL spectrum acquisition system and using carbon nitrides as monochromatic luminophores. A wavelength-resolved multiplexing ECL biosensor is demonstrated to simultaneously detect CA19-9 and mesothelin, two pancreatic cancer biomarkers, at a single-electrode interface. This work could initiate new opportunities for more general multiplex ECL biosensors with competitive performances.

中文翻译：

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耦合的荧光剂-恒电位仪系统和无金属单色发光体，用于高分辨率波长分辨电化学发光多重生物测定

多种生物标志物的灵敏同时检测对于疾病的早期诊断至关重要。与其他光学传感技术相比，电化学发光（ECL）具有突出的优势，例如背景低。但是，由于缺乏通用的ECL光谱仪，而且数十年来生物相容性单色ECL发光体的数量有限，因此阻碍了多重ECL生物测定。在这里，我们报告通过重新检查最近的台式分光光度计耦合的恒电位仪作为高分辨率ECL光谱采集系统并使用氮化碳作为单色发光体来解决这些问题。波长分辨多路ECL生物传感器被证明可以在单电极界面同时检测CA19-9和间皮素，这是两种胰腺癌生物标记物。