当前位置: X-MOL 学术Carbohydr. Polym. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Isolation, Purification and Structural Characterestics of Chondroitin Sulfate from Smooth hound Cartilage: In vitro Anticoagulant and Antiproliferative Properties
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2018-06-08 , DOI: 10.1016/j.carbpol.2018.06.040
Fatma Krichen , Hajer Bougatef , Nadhem Sayari , Federica Capitani , Ikram Ben Amor , Imed Koubaa , Francesca Maccari , Veronica Mantovani , Fabio Galeotti , Nicola Volpi , Ali Bougatef

Chondroitin sulfate was extracted from the cartilage of smooth hound (CSSH) and then purified by anion exchange chromatography. The structual characteristic of CSSH was evaluated by acetate cellulose electrophoresis, FTIR, 13C NMR and SAX-HPLC. Molecular weight of CSSH was average 68.78 KDa. Disaccharide analysis indicated that CSSH was predominately composed of monosulfated disaccharides in position 6 and 4 of the N-acetylgalactosamine (45.34% and 32.49%, respectively).

CSSH was tested for in vitro anticoagulant activity using the three classical coagulation assays (activated partial thromboplastin time (aPTT), prothrombine time (TT) and thrombin time (PT) tests). The finding showed that CSSH prolonged significatively (p < 0.05), aPTT, TT and PT about 1.4, 3.44 and 1.21 fold, respectively, greater than that of the negative control at a concentration of 100 µg/ml. The CSSH caused a significant antiproliferative activity against HCT116 cell, which was 79% of cell proliferation inhibition at the concentration of 1000 µg/ml. Further, CSSH presented no toxicity against the normal cells and no hemolysis towards bovine erythrocytes for all concentrations tested.

CSSH demonstrated hopeful antiproliferative and anticoagulant potential, which may be used as a novel and effective drug.



中文翻译:

平滑猎犬软骨中硫酸软骨素的分离,纯化和结构特征:体外抗凝和抗增殖特性

从平滑猎犬(CSSH)的软骨中提取硫酸软骨素,然后通过阴离子交换色谱法纯化。通过醋酸纤维素电泳,FTIR,13 C NMR和SAX-HPLC对CSSH的结构特征进行了评估。CSSH的平均分子量为68.78 KDa。二糖分析表明,CSSH主要由N-乙酰半乳糖胺的6和4位上的单硫酸化二糖组成(分别为45.34%和32.49%)。

使用三种经典的凝血测定法(活化的部分凝血活酶时间(aPTT),凝血酶原时间(TT)和凝血酶时间(PT)测试)对CSSH的体外抗凝活性进行了测试。该发现表明,CSSH显着延长(p <0.05),aPTT,TT和PT分别比浓度为100 µg / ml的阴性对照大1.4倍,3.44和1.21倍。CSSH对HCT116细胞产生了显着的抗增殖活性,在1000 µg / ml的浓度下,其抑制了79%的细胞增殖。此外,在所有测试浓度下,CSSH对正常细胞均无毒性,对牛红细胞也无溶血作用。

CSSH表现出令人希望的抗增殖和抗凝潜力,可以用作新型有效药物。

更新日期:2018-06-08
down
wechat
bug