当前位置: X-MOL 学术ACS Biomater. Sci. Eng. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Thermoresponsive Citrate-Based Graphene Oxide Scaffold Enhances Bone Regeneration from BMP9-Stimulated Adipose-Derived Mesenchymal Stem Cells.
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2018-06-07 , DOI: 10.1021/acsbiomaterials.8b00179
Chen Zhao 1, 2 , Zongyue Zeng 2, 3 , Nader Taheri Qazvini 4 , Xinyi Yu 1, 2 , Ruyi Zhang 2, 3 , Shujuan Yan 2, 3 , Yi Shu 2, 3 , Yunxiao Zhu 5, 6 , Chongwen Duan 5 , Elliot Bishop 7 , Jiayan Lei 1, 2 , Wenwen Zhang 2, 8 , Chao Yang 2, 3 , Ke Wu 2, 3 , Ying Wu 2, 9 , Liping An 2, 10 , Shifeng Huang 1, 2 , Xiaojuan Ji 2, 3 , Cheng Gong 11 , Chengfu Yuan 2, 12 , Linghuan Zhang 2, 3 , Wei Liu 1, 2 , Bo Huang 2, 3 , Yixiao Feng 1, 2 , Bo Zhang 2, 10 , Zhengyu Dai 2, 13 , Yi Shen 2, 14 , Xi Wang 2, 3 , Wenping Luo 2, 3 , Leonardo Oliveira 2 , Aravind Athiviraham 2 , Michael J Lee 2 , Jennifer Moriatis Wolf 2 , Guillermo A Ameer 5, 6, 15 , Russell R Reid 2, 6, 7 , Tong-Chuan He 2, 3, 6 , Wei Huang 1
Affiliation  

Effective bone tissue engineering is important to overcome the unmet clinical challenges as more than 1.6 million bone grafts are done annually in the United States. Successful bone tissue engineering needs minimally three critical constituents: osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds. Osteogenic progenitors are derived from multipotent mesenchymal stem cells (MSCs), which can be prepared from numerous tissue sources, including adipose tissue. We previously showed that BMP9 is the most osteogenic BMP and induces robust bone formation of immortalized mouse adipose-derived MSCs entrapped in a citrate-based thermoresponsive hydrogel referred to as PPCNg. As graphene and its derivatives emerge as promising biomaterials, here we develop a novel thermosensitive and injectable hybrid material by combining graphene oxide (GO) with PPCNg (designated as GO-P) and characterize its ability to promote bone formation. We demonstrate that the thermoresponsive behavior of the hybrid material is maintained while effectively supporting MSC survival and proliferation. Furthermore, GO-P induces early bone-forming marker alkaline phosphatase (ALP) and potentiates BMP9-induced expression of osteogenic regulators and bone markers as well as angiogenic factor VEGF in MSCs. In vivo studies show BMP9-transduced MSCs entrapped in the GO-P scaffold form well-mineralized and highly vascularized trabecular bone. Thus, these results indicate that GO-P hybrid material may function as a new biocompatible, injectable scaffold with osteoinductive and osteoconductive activities for bone regeneration.

中文翻译:

基于热敏柠檬酸盐的氧化石墨烯支架可增强BMP9刺激的脂肪间充质干细胞的骨再生。

有效的骨组织工程对于克服尚未解决的临床挑战非常重要,因为在美国每年进行超过160万个骨移植。成功的骨组织工程至少需要三个关键要素:骨祖细胞,成骨因子和骨诱导性/骨传导性支架。成骨祖细胞衍生自多能间充质干细胞(MSC),可从多种组织来源(包括脂肪组织)中制备。我们以前显示BMP9是成骨性最强的BMP,并且可以诱导被永生化的小鼠脂肪来源的MSC包裹在称为PPCNg的基于柠檬酸盐的热响应水凝胶中,从而形成坚固的骨骼。随着石墨烯及其衍生物成为有前途的生物材料,在这里,我们通过将氧化石墨烯(GO)与PPCNg(称为GO-P)相结合,开发了一种新型的热敏性和可注射混合材料,并表征了其促进骨骼形成的能力。我们证明了杂化材料的热响应行为得以维持,同时有效地支持了MSC的生存和增殖。此外,GO-P诱导了早期骨形成标记碱性磷酸酶(ALP),并增强了BMP9诱导的MSC中成骨调节剂和骨标记以及血管生成因子VEGF的表达。体内研究表明,困在GO-P支架中的BMP9转导的MSC形成了矿化度高和血管化程度高的小梁骨。因此,这些结果表明GO-P杂化材料可以充当一种新的生物相容性材料,
更新日期:2018-06-07
down
wechat
bug