To accelerate the vascularization of engineered tissue, an endothelial-specific fusion protein (VEGF-Fc), which consists of a human vascular endothelial growth factor (VEGF) and an immunoglobulin G Fc region, was fabricated and used to construct a bioactive interface in a porous scaffold. In this study, VEGF-Fc was immobilized on polycarprolactone (PCL) porous scaffolds by steeping, which is mediated by the hydrophobic binding of the Fc domain. The VEGF-Fc proteins were distributed stably and uniformly throughout the PCL porous scaffolds without affecting their surface morphology and mechanical properties. The immobilized VEGF-Fc activated the phosphorylation of VEGF2 receptor continuously, and further promoted the expressions of PI3K and MAPK, which effectively enhanced the adhesion and proliferation of human vascular endothelial cells (HUVECs). Furthermore, the immobilized VEGF-Fc promoted the migration of HUVECs into the scaffolds, and also enhanced the cellularization and ECM deposition in the subcutaneous implanted scaffolds in rats, which synergistically supported the vascularization of the scaffold in vivo. In view of the advantages of easy use, stability and efficiency, the VEGF-Fc fusion protein appeared to be a promising candidate for surface modification of porous scaffolds for tissue engineering.

中文翻译：

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通过VEGF-Fc修饰增强PCL多孔支架的血管生成†

为加速工程组织的血管生成，制备了由人血管内皮生长因子（VEGF）和免疫球蛋白G Fc区组成的内皮特异性融合蛋白（VEGF-Fc），并用于在血管内皮细胞中构建生物活性界面。多孔支架。在这项研究中，通过浸泡将VEGF-Fc固定在聚己内酯（PCL）多孔支架上，这是由Fc结构域的疏水结合介导的。VEGF-Fc蛋白稳定且均匀地分布在整个PCL多孔支架中，而不会影响其表面形态和机械性能。固定化的VEGF-Fc持续激活VEGF2受体的磷酸化，进一步促进PI3K和MAPK的表达，有效增强人血管内皮细胞（HUVECs）的黏附和增殖。体内。考虑到易于使用，稳定性和效率的优点，VEGF-Fc融合蛋白似乎是用于组织工程的多孔支架的表面修饰的有前途的候选者。