Abstract The effects of side-stream ammonia stripping as a pre- and post-treatment on the anaerobic mono-digestion of chicken manure were investigated in laboratory-scale continuous stirred tank reactors. Ammonia stripping columns were operated at pH 10 but different temperatures (35, 55, 70 °C). Results showed that digestate stripping at 70 °C and pH 10 got the highest ammonia removing rate (18.2–45.2%), and under these conditions, the methane yield at OLR of 9 g VS L−1 d−1 reached 0.199 L g−1 VSadded which was significantly higher than that of other digesters (0.01–0.03 L g−1 VSadded), although no improvement of methane yield was observed at OLR 3 and 6 g VS L−1 d−1. The microbial community analysis revealed that hydrolytic and fermentative bacteria dominated all bacterial communities. Methanogenic pathway of feedstock stripping digester was predominant by hydrogenotrophic Methanimicrococcus, while hydrogenotrophic/aceticlastic Methanoscarcina was dominant in the archaeal structures of the rest digesters. However, a shift from Methanosarcina to Methanoculleus was observed in R3 (digestate stripping at 70 °C/pH 10) when dominant species were inhibited and decreased in other digesters, indicating that the high methanogenic activity in R3 was maintained by shifting to methanogens with high tolerance to inhibitors.

中文翻译：

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从鸡粪厌氧消化中去除氮以增强生物甲烷化

摘要 在实验室规模的连续搅拌釜反应器中研究了侧流氨汽提作为前处理和后处理对鸡粪厌氧单消化的影响。氨汽提塔在 pH 10 下运行，但温度不同（35、55、70 °C）。结果表明，消化液汽提在 70 °C 和 pH 10 条件下氨去除率最高（18.2-45.2%），在这些条件下，OLR 9 g VS L-1 d-1 的甲烷产率达到 0.199 L g- 1 VS added 显着高于其他消化器（0.01–0.03 L g-1 VS added），尽管在 OLR 3 和 6 g VS L-1 d-1 处没有观察到甲烷产量的提高。微生物群落分析表明，水解和发酵细菌在所有细菌群落中占主导地位。原料汽提消化池的产甲烷途径以氢营养型甲烷微球菌为主，而其余消化池的古菌结构中以氢营养型/醋酸菌为主。然而，当优势物种在其他消化器中被抑制和减少时，在 R3 中观察到从 Methanosarcina 到 Methanoculleus 的转变（在 70 °C/pH 10 消化液汽提），这表明 R3 中的高产甲烷活性是通过转移到高产甲烷菌来维持的。对抑制剂的耐受性。