Lipases from Thermomyces lanuginosus (TLL) and Burkholderia cepacia (BCL) were immobilised on desilicated and thiol-modified ZSM-5 via covalent binding. These biocatalysts were tested, both alone and combined, in a reaction for the production of fatty acid ethyl esters from macauba pulp oil. A higher yield of esters was obtained with TLL combined with BCL, and the synergic effect was investigated systematically. Under the best conditions for the reaction, using a mixture of lipases (1% water content, 3:1 M ratio of ethanol to oil, at 30°C), the yield of ethyl esters was 93.1% ± 1.4% over48 h. The mixed lipases could be used in six operation cycles with a retained relative activity of higher than 60%. The combined biocatalysts were also evaluated using reactions carried out with macauba pulp oils with lower free fatty acid content, resulting in lower yields than those obtained with the initial oil.

来自兰热霉菌（TLL）和洋葱伯克霍尔德氏菌的脂肪酶（BCL）通过共价结合固定在脱硅的和巯基修饰的ZSM-5上。在由马卡巴纸浆油生产脂肪酸乙酯的反应中，单独或组合地测试了这些生物催化剂。TLL与BCL联合使用可获得较高的酯收率，并系统地研究了其协同作用。在最佳的反应条件下，使用脂肪酶混合物（水含量为1％，乙醇与油的比例为30：3，在30°C，在30°C），在48小时内乙酯的收率为93.1％±1.4％。混合脂肪酶可用于六个操作周期，保留的相对活性高于60％。还使用与具有较低游离脂肪酸含量的马卡巴纸浆油进行的反应来评估组合的生物催化剂，从而导致收率低于用初始油获得的收率。