The frequent occurrence of Microcystis aeruginosa blooms benefit from the dormant Microcystis cells, which will be recruited from sediment into overlying water to form a dominant population and algal blooms when external environmental conditions are suitable. Previous studies have unveiled factors involved in M. aeruginosa recruitment and bloom initiation, including nutrition, illumination, temperature, and hydrodynamic force. In this study, three dominant benthic bacterial species isolated from Lake Chongtian with frequent blooms-forming were identified through next generation sequencing (NGS) techniques, and laboratory experiments were conducted on the recruitment of dormant M. aeruginosa cells via co-culture with these bacteria at 10 °C, 15 °C, 20 °C and 25 °C. The results showed that the bacterial strains in sediment proliferated quickly before recruitment of dormant M. aeruginosa cells, subsequently significantly promoted the recruitment of dormant M. aeruginosa via allelochemical (metabolite) production, lower N:P values and lower dissolved oxygen concentrations in the sediment-water interface, and enhanced photosynthesis of M. aeruginosa cells. Furthermore, dormant M. aeruginosa was recruited from sediment at 10 °C when bacterial activity was present, but not recruited when bacterial activity was absent. At 15 °C,20 °Cand 25 °C, there were no remarkable differences in the recruitment rate of dormant M. aeruginosa cells among all bacterial groups, although their recruitment rate were significantly higher than that at 10 °C.These findings suggested that, under laboratory conditions, three benthic bacteria not only had a great influence on promoting the recruitment of dormant M. aeruginosa cells under desirable temperatures, but also can spur recruitment of dormant M. aeruginosacells from sediment at lower temperature (10 °C).

休眠的微囊藻细胞受益于铜绿微囊藻绽放的频繁发生，当外部环境条件合适时，这些微囊藻细胞将从沉积物中募集到上层水中，以形成优势种群和藻华。先前的研究揭示了铜绿假单胞菌募集和花序萌发的相关因素，包括营养，光照，温度和流体动力。在这项研究中，三大主导底栖细菌物种冲天湖分离频繁的花朵形成是通过下一代测序鉴定（NGS）技术，和实验室实验在休眠的招聘进行微囊藻细胞通过在10°C，15°C，20°C和25°C下与这些细菌共培养。结果表明，沉积物中的细菌菌株在募集休眠的铜绿假单胞菌细胞之前迅速增殖，随后通过化肥（代谢物）产生，较低的N：P值和沉积物中的溶解氧浓度显着促进了休眠的铜绿假单胞菌的募集。-水界面，增强铜绿假单胞菌细胞的光合作用。此外，当存在细菌活性时，休眠的铜绿假单胞菌是在10°C时从沉积物中募集的，而当没有细菌活性时则不募集休眠的铜绿假单胞菌。在15°C，20°C和25°C时，休眠的募集率没有显着差异尽管所有细菌组中的铜绿假单胞菌细胞的募集率均明显高于10°C，但这些结果表明，在实验室条件下，三种底栖细菌不仅对促进休眠的铜绿假单胞菌的募集有很大的影响。细胞在理想的温度下，但也可以刺激较低温度（10°C）的沉积物中募集休眠的铜绿假单胞菌细胞。