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Ultrastructural Imaging of Salmonella–Host Interactions Using Super‐resolution Correlative Light‐Electron Microscopy of Bioorthogonal Pathogens
ChemBioChem ( IF 3.2 ) Pub Date : 2018-07-05 , DOI: 10.1002/cbic.201800230
Daphne M van Elsland 1, 2 , Sílvia Pujals 3 , Thomas Bakkum 1 , Erik Bos 4 , Nikolaos Oikonomeas-Koppasis 1 , Ilana Berlin 2 , Jacques Neefjes 2 , Annemarie H Meijer 5 , Abraham J Koster 4 , Lorenzo Albertazzi 3, 6 , Sander I van Kasteren 1
Affiliation  

Bioorthogonally labelled intracellular pathogens are visualized within an infected immune cell by using a technique called STORM‐CLEM. By using super‐resolution microscopy in combination with transmission electron microscopy, super‐resolution fluorescence information of the pathogen can be positioned on the ultrastructural context of the infected cell.
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中文翻译:

使用生物正交病原体的超分辨率相关光电子显微镜对沙门氏菌与宿主相互作用进行超微结构成像

通过使用 STORM‐CLEM 技术,可以在受感染的免疫细胞内可视化生物正交标记的细胞内病原体。通过使用超分辨率显微镜与透射电子显微镜相结合,可以将病原体的超分辨率荧光信息定位在受感染细胞的超微结构背景上。
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更新日期:2018-07-05
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