All analytical techniques are governed by the principles of physical chemistry, and chromatography is certainly no exception. By examining the underlying concepts of size exclusion chromatography (SEC), students can gain greater appreciation of and insight into the science and utility of thermodynamics, as well as solution properties of macromolecules. In this paper we will show how the separation mechanism that governs SEC, an unusual form of chromatography, can be explained using the tenets of thermodynamics. This paper gives educators access to an HPLC technique usually not included in undergraduate chemistry curriculum.

中文翻译：

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尺寸排阻色谱法：物理化学的教学辅助

所有分析技术均受物理化学原理的支配，色谱法当然也不例外。通过研究尺寸排阻色谱法（SEC）的基本概念，学生可以对热力学的科学和效用以及大分子的溶液特性有更深入的了解和了解。在本文中，我们将展示如何使用热力学原理来解释支配SEC的分离机制（一种不寻常的色谱形式）。本文使教育工作者可以使用通常不包括在本科化学课程中的HPLC技术。