当前位置: X-MOL 学术ChemistrySelect › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Photocontrollable Fluorogenic Probe for Visualizing Near‐Membrane Hypochlorite in Live Cells
ChemistrySelect ( IF 2.1 ) Pub Date : 2018-06-04 , DOI: 10.1002/slct.201800777
Pingping Peng 1 , Hao Li 1 , Lei Bai 1 , Liulin Wang 1 , Buxiang Chen 1 , Changmin Yu 1 , Chengwu Zhang 1 , Jingyan Ge 2 , Lin Li 1 , Wei Huang 1, 3
Affiliation  

Hypochlorous acid or hypochlorite (HClO/ClO) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane‐anchoring unit (cholesterol) and ClO‐sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO. However, no fluorescence was observed with the treatment of only UV irradiation or ClO. In addition, this new probe displayed a good linear relationship with the concentration of ClO ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO over other analytes. Furthermore, cell‐based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near‐membrane ClO with light switching on/off.

中文翻译:

光控荧光探针可视化活细胞中的近膜次氯酸盐

次氯酸或次氯酸盐(高氯酸/ CLO - )附近膜是最关键的活性氧物质(ROS)中的一个。在本文中,用于直接检测CLO一个photocontrollable荧光探针-近膜已经设计并合成。该探针HOCMem是基于通过光不稳定的基团(硝基苄基),膜-锚定单元(胆固醇)和C10的掺入荧光素构建-敏感的部分(苯甲酰基)。当UV照射时,探针显示出在CLO的存在520nm的显著增加发射- 。但是,仅用紫外线照射或ClO-处理,未观察到荧光。此外,这种新的探针显示的良好的线性与CLO的浓度关系-介于1至50μM与260.77 nM的检测极限,以及用于CLO优异的选择性-在其他分析物。此外,基于细胞的实验显示这种探针及其用于直接检测的近膜CLO容量的低细胞毒性-光开关的开/关。
更新日期:2018-06-04
down
wechat
bug