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Photocontrollable Fluorogenic Probe for Visualizing Near‐Membrane Hypochlorite in Live Cells
ChemistrySelect ( IF 2.1 ) Pub Date : 2018-06-04 , DOI: 10.1002/slct.201800777 Pingping Peng 1 , Hao Li 1 , Lei Bai 1 , Liulin Wang 1 , Buxiang Chen 1 , Changmin Yu 1 , Chengwu Zhang 1 , Jingyan Ge 2 , Lin Li 1 , Wei Huang 1, 3
ChemistrySelect ( IF 2.1 ) Pub Date : 2018-06-04 , DOI: 10.1002/slct.201800777 Pingping Peng 1 , Hao Li 1 , Lei Bai 1 , Liulin Wang 1 , Buxiang Chen 1 , Changmin Yu 1 , Chengwu Zhang 1 , Jingyan Ge 2 , Lin Li 1 , Wei Huang 1, 3
Affiliation
Hypochlorous acid or hypochlorite (HClO/ClO−) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO− near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane‐anchoring unit (cholesterol) and ClO−‐sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO−. However, no fluorescence was observed with the treatment of only UV irradiation or ClO−. In addition, this new probe displayed a good linear relationship with the concentration of ClO− ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO− over other analytes. Furthermore, cell‐based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near‐membrane ClO− with light switching on/off.
中文翻译:
光控荧光探针可视化活细胞中的近膜次氯酸盐
次氯酸或次氯酸盐(高氯酸/ CLO - )附近膜是最关键的活性氧物质(ROS)中的一个。在本文中,用于直接检测CLO一个photocontrollable荧光探针-近膜已经设计并合成。该探针HOCMem是基于通过光不稳定的基团(硝基苄基),膜-锚定单元(胆固醇)和C10的掺入荧光素构建-敏感的部分(苯甲酰基)。当UV照射时,探针显示出在CLO的存在520nm的显著增加发射- 。但是,仅用紫外线照射或ClO-处理,未观察到荧光。。此外,这种新的探针显示的良好的线性与CLO的浓度关系-介于1至50μM与260.77 nM的检测极限,以及用于CLO优异的选择性-在其他分析物。此外,基于细胞的实验显示这种探针及其用于直接检测的近膜CLO容量的低细胞毒性-光开关的开/关。
更新日期:2018-06-04
中文翻译:
光控荧光探针可视化活细胞中的近膜次氯酸盐
次氯酸或次氯酸盐(高氯酸/ CLO - )附近膜是最关键的活性氧物质(ROS)中的一个。在本文中,用于直接检测CLO一个photocontrollable荧光探针-近膜已经设计并合成。该探针HOCMem是基于通过光不稳定的基团(硝基苄基),膜-锚定单元(胆固醇)和C10的掺入荧光素构建-敏感的部分(苯甲酰基)。当UV照射时,探针显示出在CLO的存在520nm的显著增加发射- 。但是,仅用紫外线照射或ClO-处理,未观察到荧光。。此外,这种新的探针显示的良好的线性与CLO的浓度关系-介于1至50μM与260.77 nM的检测极限,以及用于CLO优异的选择性-在其他分析物。此外,基于细胞的实验显示这种探针及其用于直接检测的近膜CLO容量的低细胞毒性-光开关的开/关。