Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2018-06-04 , DOI: 10.1016/j.carbpol.2018.06.003 Suzanne E. Broxterman , Henk A. Schols
The primary plant cell wall is composed of a complex network of pectin, hemicellulose and cellulose. Potential interactions between these polysaccharides were studied for carrot, tomato and strawberry, with a focus on the role of pectin. The Chelating agent Unextractable Solids (ChUS), the residue after water- and EDTA extraction, was ball milled and subsequently water extracted. For tomato and strawberry, pectin and substantial amounts of hemicellulose were solubilised. Anion exchange chromatography (AEC) showed co-elution of pectin and acetylated glucuronoxylan in tomato, representing 18% of solubilised uronic acid and 48% of solubilised xylose by ball milling from ChUS. The existence of a covalently linked pectin-xylan complex was proposed since xylan co-precipitated with pectin under mild alkali conditions. It was proposed that pectin links with xylan through the RG-I region since degradation of HG did not alter AEC elution patterns for RG-I and xylan, suggesting RG-I − xylan interactions.
中文翻译:
番茄原生植物细胞壁中果胶-木聚糖复合物的表征
主要的植物细胞壁由果胶,半纤维素和纤维素的复杂网络组成。研究了胡萝卜,番茄和草莓中这些多糖之间的潜在相互作用,重点是果胶的作用。将水和EDTA萃取后的残留物螯合剂不可萃取固体(ChUS)进行球磨,然后用水萃取。对于番茄和草莓,可溶解果胶和大量的半纤维素。阴离子交换色谱(AEC)显示,通过ChUS球磨,可将番茄中的果胶和乙酰化葡萄糖醛酸木聚糖共洗脱,分别代表18%的可溶性糖醛酸和48%的可溶性木糖。提出了共价连接的果胶-木聚糖复合物的存在,因为木聚糖在温和的碱性条件下与果胶共沉淀。