Tetrachlorobisphenol A (TCBPA), which is widely used as flame retardant, can be released into various environments, thereby being absorbed by wildlife or human beings through food chain's bio-magnification and causing some adverse influences on wildlife or human beings. However, limited data are currently available on TCBPA-associated cytotoxicity and related mechanisms. Here, the cytotoxicity induced by different concentrations of TCBPA (i.e., 5, 10 and 20 μM) was studied using Saccharomyces cerevisiae, a simple eukaryotic model organism. TCBPA treatment inhibited the growth and survival rate of yeast cell in a dose-dependent manner. Moreover, TCBPA promoted the increasing of intracellular oxidative stress by enhancing accumulation of intracellular reactive oxygen species (ROS). Meanwhile, lipid peroxidation degree (represented by malondialdehyde (MDA) content) and DNA damage degree (represented by 8-hydroxy deoxyguanosine (8-oxodG) content) in yeast cell also increased after TCBPA treatment. However, yeast cell mitochondrial membrane potential (Δψm) decreased after TCBPA treatment. It was noteworthy that there was no significant inhibitory effect on yeast cell growth or survival rate in 5 μM TCBPA-treated cells, but the intracellular MDA content and Δψm level changed significantly, suggesting the potential cell damage secondary to the relative low dose of TCBPA exposure. Results presented here would highlight our knowledge about TCBPA-associated cytotoxicity in organisms.

广泛用作阻燃剂的四氯双酚A（TCBPA）可以释放到各种环境中，从而通过食物链的生物放大作用而被野生生物或人类吸收，并对野生生物或人类造成一些不利影响。但是，有关TCBPA相关的细胞毒性和相关机制的可用数据有限。在这里，使用酿酒酵母研究了不同浓度的TCBPA（即5、10和20μM）诱导的细胞毒性，一种简单的真核生物模型。TCBPA处理以​​剂量依赖性方式抑制酵母细胞的生长和存活率。此外，TCBPA通过增强细胞内活性氧（ROS）的积累来促进细胞内氧化应激的增加。同时，TCBPA处理后，酵母细胞中脂质过氧化程度（以丙二醛（MDA）含量表示）和DNA损伤程度（以8-羟基脱氧鸟苷（8-oxodG）含量表示）也增加。但是，TCBPA处理后，酵母细胞线粒体膜电位（Δψm）降低。值得注意的是，经5μMTCBPA处理的细胞对酵母细胞的生长或存活率没有明显的抑制作用，但细胞内MDA含量和Δψm水平却发生了显着变化，提示相对较低剂量的TCBPA暴露会继发潜在的细胞损伤。此处提供的结果将突出我们对生物体内与TCBPA相关的细胞毒性的了解。