Regarding the humane use of animals in scientific research, invertebrates are often recommended in toxicological studies. “Freshwater planarians” refers to numerous free-living freshwater members of the Class “Turbellaria” of the phylum Platyhelminthes. This group of invertebrates has received extensive attention from biologists for many years because of their unique biological characteristics, such as the primitive form of the central nervous system and notable capability to regenerate tissues. Using freshwater planarians as test animals in chemical toxicity studies has grown in popularity since the 1960s. Results from various toxicological experiments have collectively suggested that freshwater planarians can serve as not only alternative models for chemical toxicity screenings in laboratories but also as potential bioindicators for the quality of freshwater environments. However, thus far, no standardized battery of tests for conducting toxicological studies that includes freshwater planarians has been proposed. This paper comprehensively reviews the toxicological information obtained from chemically exposed planarians and proposes practical factors for consideration in toxicity experiments with freshwater planarians as test organisms.

关于科学研究中动物的人道使用，通常在毒理学研究中建议使用无脊椎动物。“淡水浮游动物”是指鸭嘴兽门的“ Turbellaria”类的许多自由生活的淡水成员。这组无脊椎动物由于其独特的生物学特性（例如中枢神经系统的原始形式和显着的组织再生能力）而受到生物学家多年的广泛关注。自从1960年代以来，在化学毒性研究中使用淡水浮游生物作为试验动物的现象已日益普及。各种毒理学实验的结果共同表明，淡水涡虫不仅可以作为实验室化学毒性筛选的替代模型，而且可以作为淡水环境质量的潜在生物指标。然而，到目前为止，尚未提出用于进行包括淡水浮游动物在内的毒理学研究的标准测试组合。本文全面回顾了从化学暴露的平面虫获得的毒理学信息，并提出了在以淡水平面虫为测试生物的毒性实验中要考虑的实际因素。