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Metagenomic insights into the effect of oxytetracycline on microbial structures, functions and functional genes in sediment denitrification
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2018-06-02 , DOI: 10.1016/j.ecoenv.2018.05.045
Yong Zou , Manxia Lin , Wenguang Xiong , Mei Wang , Jiaxuan Zhang , Mianzhi Wang , Yongxue Sun

Denitrification is an indispensable pathway of nitrogen removal in aquatic ecosystems, and plays an important role in decreasing eutrophication induced by excessive reactive nitrogen pollution. Aquatic environments also suffer from antibiotic pollution due to runoff from farms and sewage systems. The aim of this study was to investigate the effect of oxytetracycline stress on denitrifying functional genes, the microbial community and metabolic pathways in sediments using high-throughput sequencing and metagenomic analysis. The oxytetracycline was observed to significantly inhibit the abundance of nirK and nosZ genes (P < 0.001). KEGG pathway annotation indicated that oxytetracycline treatment decreased the abundance of nitrate reductase, nitrite reductase and N2O reductase. Functional annotations revealed that oxytetracycline exposure decreased the abundance of the protein metabolism subsystem in the bacterial community. Metagenomic sequencing demonstrated that the abundance of Proteobacteria and Firmicutes increased with oxytetracycline exposure while the Actinobacteria decreased. In sediments, Pseudomonas and Bradyrhizobium were major contributors to denitrification and oxytetracycline exposure resulted in a decreased abundance of Bradyrhizobium. These results indicated that oxytetracycline residues influences the denitrifier community and may heighten occurrence of reactive nitrogen in aquatic ecosystems.



中文翻译:

土霉素对沉积物反硝化过程中微生物结构,功能和功能基因的影响的元基因组学见解

反硝化是水生生态系统中氮去除的必不可少的途径,并且在减少因过量反应性氮污染而引起的富营养化中起着重要作用。由于农场和污水系统的径流,水生环境还遭受抗生素污染。这项研究的目的是使用高通量测序和宏基因组分析来研究土霉素对脱氮功能基因,微生物群落和代谢途径的影响。土霉素被认为可以显着抑制nirKnosZ基因的丰度(P <0.001)。KEGG通路注释表明,土霉素的处理降低了硝酸还原酶,亚硝酸还原酶和N 2的丰度O还原酶。功能注释表明,土霉素的暴露降低了细菌群落中蛋白质代谢子系统的丰度。元基因组测序表明,随着土霉素的暴露,变形杆菌坚定菌的丰度增加,而放线菌则减少。在沉积物中,假单胞菌根瘤菌是主要贡献者脱氮和土霉素暴露导致了丰下降根瘤菌。这些结果表明,土霉素残留影响反硝化器的群落,并可能增加水生生态系统中反应性氮的含量。

更新日期:2018-06-02
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