Three O. volvulus immunogenic peptide sequences recently discovered by peptide microarray were adapted to a lateral flow assay (LFA). The LFA employs gold nanoshells as novel high-contrast reporter nanoparticles and detects a serological response against the 3 peptides, found in OvOC9384, OvOC198, and OvOC5528, respectively. When tested on 118 sera from O. volvulus infected patients and 208 control sera, the LFA was 90%, 63%, and 98% sensitive for each peptide, respectively, and 99–100% specific vs samples from healthy volunteers. Samples of other filarial infections cross-reacted by 7–24%. The sensitivity, specificity, and cross-reactivity values matched those obtained by ELISA with the same sample set. While the exact choice of peptide(s) will require fine-tuning, this work establishes that O. volvulus peptides identified by peptide microarray can be translated into an antibody-based LFA and that gold nanoshells provide the same sensitivity, specificity, and cross-reactivity as the corresponding ELISA assays.

中文翻译：

---

盘尾丝虫病的概念验证快速诊断测试：肽生物标志物研究和金纳米壳作为报告纳米颗粒的使用

最近通过肽微阵列发现的三种食蟹曲霉免疫原性肽序列适用于侧向流动测定（LFA）。LFA使用金纳米壳作为新型高对比度报道分子，并检测针对分别在OvOC9384，OvOC198和OvOC5528中发现的3种肽的血清学应答。当在食蟹肠杆菌的118种血清中检测时被感染的患者和208个对照血清，LFA对每种肽的敏感性分别为90％，63％和98％，与健康志愿者的样品相比，其特异性为99-100％。其他丝状感染的样本交叉反应7–24％。灵敏度，特异性和交叉反应性值与相同样品组的ELISA值相匹配。尽管要精确选择一种或多种肽，但这项工作确定了可以将通过肽微阵列鉴定的肠螺旋体肽翻译成基于抗体的LFA，并且金纳米壳具有相同的敏感性，特异性和交叉性。作为相应的ELISA分析的反应性。