Globularia alypum L. (GA) is a mediterranean plant of the Globulariaceae family. GA aqueous extract (GAAE) is widely used to treat various ailments, including constipation, peptic ulcer, diabetes, insomnia, hernia and intestinal pain. In this study we analyzed the chemical composition of GAAE by HPLC-PDA/ESI-MS and we examined its protective effect on acetic acid (AA)-induced ulcerative colitis in rats. We firstly showed that the HPLC-PDA/ESI-MS analysis indicated high concentrations of secondary metabolites in GAAE, especially iridoids. We have also shown that rectal administration of AA provoked a severe colon mucosal injury and macroscopic edematous colon inflammation with alteration of colonic oxidative balance. However GAAE pretreatment protected significantly and dose dependently against all colonic morphological, histological and biochemical alterations induced by AA. This study suggests that GAAE possess active compounds, which exert marked protective effects in experimental colitis, possibly by regulating the colonic epithelium inflammatory mediators, oxidative balance and calcium homeostasis.

球根L.（GA）是globulariaceae家族的地中海植物。GA水提取物（GAAE）被广泛用于治疗各种疾病，包括便秘，消化性溃疡，糖尿病，失眠，疝气和肠道疼痛。在这项研究中，我们通过HPLC-PDA / ESI-MS分析了GAAE的化学成分，并研究了其对乙酸（AA）诱导的大鼠溃疡性结肠炎的保护作用。我们首先表明，HPLC-PDA / ESI-MS分析表明GAAE中的次级代谢产物浓度很高，尤其是虹膜类化合物。我们还表明，直肠给予AA会引起严重的结肠粘膜损伤和肉眼可见的水肿性结肠炎症，并改变结肠的氧化平衡。但是，GAAE预处理可显着保护并剂量依赖性地抵御所有结肠形态，AA诱导的组织学和生化改变。这项研究表明，GAAE具有活性化合物，可能通过调节结肠上皮炎性介质，氧化平衡和钙稳态来在实验性结肠炎中发挥显着的保护作用。