To elucidate the anorexigenic action and inhibitory effect of fat accumulation by epigallocatechin gallate (EGCG) and caffeine, including the optimal combination ratio and mechanism, fifteen diets with several concentrations of EGCG and/or caffeine were administered to mice for eight weeks. The 0.1% EGCG + 0.1% caffeine group showed the strongest suppression of food intake and a remarkable reduction of body weight and fat accumulation; therefore, the ratio was determined to be the optimal combination ratio. Moreover, serum glucagon-like peptide-1 (GLP-1) level and hypothalamic gene expression of pro-opiomelanocortin (POMC) were promoted by 0.1% EGCG + 0.1% caffeine. In conclusion, the combined treatment of 0.1% EGCG + 0.1% caffeine induces not only suppression of fat accumulation but also strong anorexigenic action in mice. The anorexigenic effect may be brought about via inhibiting gastric motility by GLP-1 and upregulating POMC in the hypothalamus.

为了阐明表没食子儿茶素没食子酸酯（EGCG）和咖啡因对脂肪蓄积的厌食作用和抑制作用，包括最佳的组合比例和机理，对小鼠进行了15种含几种浓度的EGCG和/或咖啡因的饮食，共8周。0.1％EGCG + 0.1％咖啡因组显示出对食物摄入的最强抑制作用，并显着降低了体重和脂肪堆积；因此，将该比率确定为最佳组合比率。此外，0.1％EGCG + 0.1％咖啡因可促进血清胰高血糖素样肽-1（GLP-1）的水平和下丘脑基因原促黑素皮质激素（POMC）的表达。总之，0.1％EGCG + 0.1％咖啡因的联合治疗不仅可以抑制脂肪堆积，还可以增强小鼠的厌食作用。