A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L) in the ascending colon; acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon; propionate (+4.1 mmol/L) in the descending colon. The treatment led to a decrease in ammonium and increased lactate levels in the ascending colon, which correlated with an increase in lactobacilli and bifidobacteria (+2.5 Log/mL). Additionally, the propionate-producing Akkermansia muciniphila increased in the lumen of the proximal colon (up to 7.15 log/mL). Finally, it was observed a protective effect on Caco-2 barrier function in the distal colon and pronounced immune activating properties. The generated data support a possible role in protecting against obesity-related adverse effects.

经过验证的体外肠道模型（即SHIME®）与上皮细胞和巨噬细胞的共培养物一起用于研究低聚木糖/多酚混合物长期服用的益生元潜力和免疫调节作用。共混物在整个结肠中充分发酵，导致上升结肠中丁酸酯的产量增加（+7 mmol / L）。横结肠中的乙酸根，丙酸根和丁酸根（各约+4 mmol / L）；降结肠中的丙酸酯（+4.1 mmol / L）。该治疗导致上升结肠中的铵减少和乳酸水平升高，这与乳酸杆菌和双歧杆菌的增加（+2.5 Log / mL）相关。此外，生产丙酸的阿克曼曼丝菌在近端结肠腔中增加（高达7.15 log / mL）。最后，观察到对远端结肠中Caco-2屏障功能的保护作用和明显的免疫激活特性。生成的数据在预防与肥胖相关的不良影响方面起着可能的作用。