Lactobacillus fermentum TcUESC01 (LF) and Lactobacillus plantarum TcUESC02 (LP) isolated from the fermentation of cocoa (Theobroma cacao L.) were evaluated as probiotics. In a first step, both lactobacilli were tested for in vitro antagonism against six pathogenic bacteria. In a second step using mice orally challenged with Salmonella Typhimurium, a protective effect of administration of the lactobacilli was evaluated by determination of survival, translocation to spleen and liver, IgA levels in intestinal fluid, pro- and anti-inflammatory expression of cytokines in ileum, and histological and morphometric analysis in ileum and liver. LF and LP showed to be potential probiotics as demonstrated by large spectrum of antagonism against enteropathogens in the in vitro evaluation, as well as by the higher survival after enteropathogen challenge, lower hepatic and splenic translocation of enteropathogen, higher IgA ileal production, lower histopathological lesions in liver and anti-inflammatory pattern of immunological response in the in vivo experimental infection.

发酵乳杆菌TcUESC01（LF）和植物乳杆菌TcUESC02（LP）从可可发酵（隔绝可可L.）被评价为益生菌。第一步，测试了两种乳杆菌对六种病原菌的体外拮抗作用。在第二步中，使用经沙门氏菌口服攻击的小鼠通过确定存活率，脾脏和肝脏易位，肠液中IgA水平，回肠中细胞因子的促炎和抗炎表达以及回肠和肝中的组织学和形态学分析来评估鼠伤寒，给予乳杆菌的保护作用。LF和LP被证明是潜在的益生菌，在体外评估中对肠病原体具有广泛的拮抗作用，在肠病原体攻击后存活率较高，肠病原体肝和脾移位较低，IgA回肠产生较高，组织病理学病变较低，从而证明了LF和LP是潜在的益生菌。在肝脏中的抗炎和抗炎模式在体内实验中的免疫反应。