A simple and effective platform that can conglomerate various microfluidic functions in a single chip is essential for many bioassays, especially for point-of-care testing applications. Here, a chip that exploits surface tension in solutions with movable top and bottom layers is presented, for use in fluid transport, mixing, maintaining metered volumes, and biomolecule capture and release. The chip has open chambers in vertically mobile top layers and rotationally mobile bottom layers to exploit surface tension in biochemical solutions, and implements control over fluid motion. To manipulate biomolecules, a vertically mobile tip with a permanent magnet at the top layer performs collection, transport, release, and dispersion of magnetic beads. Thus, the chip orchestrates various fluidic control functions without using on-chip valves and pumps that increase operational and structural complexity. To demonstrate its utility, the chip performs automated DNA extraction by obtaining genomic DNA from a sample containing cells. Our approach provides a useful and effective alternative to numerous platforms that use active and passive on-chip components for bioassays.

中文翻译：

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带有可移动层的微流控芯片，用于生化试剂的处理†

一个简单有效的平台可以在单个芯片中整合各种微流体功能，对于许多生物测定，尤其是即时检验应用而言，必不可少。在这里，提出了一种在具有可移动顶层和底层的溶液中利用表面张力的芯片，该芯片可用于流体传输，混合，保持计量体积以及生物分子捕获和释放。该芯片在垂直移动的顶层和旋转移动的底层中具有开放腔室，以利用生化溶液中的表面张力，并实现对流体运动的控制。为了操纵生物分子，在顶层具有永久磁铁的垂直移动尖端可进行磁珠的收集，运输，释放和分散。因此，该芯片可协调各种流体控制功能，而无需使用片上阀门和泵，从而增加了操作和结构的复杂性。为了证明其实用性，该芯片通过从含有细胞的样品中获得基因组DNA来进行DNA自动提取。我们的方法为使用主动和被动芯片上组件进行生物测定的众多平台提供了一种有用且有效的替代方法。