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Ethanol effect on gold nanoparticle aggregation state and its implication in the interaction mechanism with DNA
Journal of Colloid and Interface Science ( IF 9.9 ) Pub Date : 2018-05-31 , DOI: 10.1016/j.jcis.2018.05.108
Elia Grueso , Pilar Perez-Tejeda , R.M. Giráldez-Pérez , R. Prado-Gotor , F. Muriel-Delgado

The equilibria and kinetics aspects of the binding of small gold nanoparticles, AuNPs, stabilized with tiopronin to DNA in B and C conformation (B-DNA and C-DNA), has been investigated in ethanol/water mixtures using different techniques. Two modes of binding are displayed: groove binding and partial intercalation, depending on the ethanol content, [EtOH], and the molar ratio, R = CAuNPs/CDNA. Two reaction mechanisms are proposed for AuNPs/DNA interaction in each polymer conformation, and the reaction parameters are evaluated. For lower ethanol levels, ([EtOH] up to 30%), when DNA is in the B form, the simplest mechanism according to the kinetic and thermodynamic results proved to be a three-step series mechanism reaction scheme which evolves in the formation of the groove complex. In this context, solvent hydration as well as the solvent effective viscosity are the main factors that influence kinetics. In contrast, for high ethanol levels, when DNA is in a C-like conformation, the mechanism is more complex involving three parallel reactions, in which AuNPs self-aggregation plays a key role in the switch from partial intercalation to groove binding. On the whole, it is evident that AuNPs aggregation and the DNA conformation are two key factors that must be taken into account in order to control the mechanism of AuNPs/DNA interaction.



中文翻译:

乙醇对金纳米粒子聚集态的影响及其与DNA相互作用的机理

在乙醇/水混合物中使用不同的技术研究了用硫普罗宁稳定的小金纳米颗粒AuNP与B和C构象(B-DNA和C-DNA)结合的平衡和动力学方面。显示了两种结合模式:凹槽结合和部分插入,具体取决于乙醇含量[EtOH]和摩尔比,R = C AuNPs / C DNA。针对每种聚合物构象中的AuNPs / DNA相互作用,提出了两种反应机理,并对反应参数进行了评估。对于较低的乙醇水平([EtOH]高达30%),当DNA为B形式时,根据动力学和热力学结果,最简单的机理被证明是一个三步串联机理反应方案,该反应方案随着反应的形成而发展。凹槽复合体。在这种情况下,溶剂水合以及溶剂有效粘度是影响动力学的主要因素。相反,对于高乙醇含量,当DNA呈C样构象时,其机理更为复杂,涉及三个平行反应,其中AuNPs的自聚集在从部分插入到沟槽结合的转换中起关键作用。总体上,

更新日期:2018-05-31
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