Past research showed a strong linear correlation between levels of the mycotoxins lolitrem B (LB, a tremorgen) and ergovaline (EV, an ergot alkaloid and potent vasoconstrictor) in perennial ryegrass (PRG) forage. The purpose of this study was to characterize the excretion of these two compounds in beef cattle consuming PRG straw and to utilize liquid chromatography–tandem mass spectrometry to investigate the metabolism of LB and EV in excreta. Four groups of steers (n = 6/group) were fed endophyte-infected PRG for 64 days (2256/638, 1554/373, 1012/259, or 247/<100 μg/kg LB/EV). Concentrations of LB and EV in both PRG straw and feces showed a linear relationship to each other. Feces reflected a dose–response for both mycotoxins, with values increasing most rapidly through 21 days then plateauing. Urine contained no detectable level of either compound or the ergoline lysergic acid. Screening for metabolites showed oxidation and reduction biotransformations for both toxins, with additional conjugation products detected for ergovaline.

中文翻译：

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共同饲喂多年生黑麦草（黑麦草）秸秆中的霉菌毒素Lolitrem B和麦角花碱：排泄物的代谢特征

过去的研究表明，多年生黑麦草（PRG）饲草中的霉菌毒素lolitrem B（LB，一种颤动素）和ergovaline（EV，一种麦角生物碱和有效的血管收缩剂）的水平之间具有很强的线性相关性。这项研究的目的是表征消耗PRG秸秆的肉牛中这两种化合物的排泄，并利用液相色谱-串联质谱法研究排泄物中LB和EV的代谢。四组of牛皮（n= 6 /组）喂食内生细菌感染的PRG 64天（2256 / 638、1554 / 373、1012 / 259或247 / <100μg/ kg LB / EV）。PRG稻草和粪便中的LB和EV浓度彼此呈线性关系。粪便反映了两种霉菌毒素的剂量-反应，其值在21天达到平稳期之后增长最快。尿液中不含可检测水平的化合物或麦角灵麦角麦角酸。代谢产物的筛选显示了两种毒素的氧化和还原生物转化，以及检测到的麦角新碱偶联产物。