Here, we have developed a new strategy to stabilize i-motif DNA in neutral and alkaline media by incorporating C-rich sequences inside silica nano-channels. Subsequently, the reversibility of this conformational transition has been achieved using a positively charged protein. Importantly, this entire conformational transition can be performed in multiple cycles, which offers an alternative way to control i-motif formation other than pH and thermal annealing.

中文翻译：

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二氧化硅纳米通道在中性和碱性pH值下诱导的i-基序形成和稳定†

在这里，我们开发了一种新的策略，可以通过将富含C的序列整合到二氧化硅纳米通道中来稳定中性和碱性介质中的i-motif DNA。随后，已经使用带正电的蛋白质实现了这种构象转变的可逆性。重要的是，可以在多个循环中执行整个构象转变，这提供了控制pH值和热退火以外的其他方法来控制i-基序的形成。