Quorum sensing (QS) is crucial for bacterial survival and activity. Although detecting related signaling metabolites can reveal QS, a versatile platform for convenient real-time imaging of their secretion in the context of bacterial biofilms along with inhibition to the growth of biofilms is still highly desired. Here we develop a flexible sticky note with a sandwich structure by encapsulating gold nanostars between two pieces of hexagonal boron nitride layers, which can be easily pasted on natural biofilms to monitor in real-time the secreted signaling molecule by SERS imaging with high sensitivity and spatiotemporal resolution. Using Pseudomonas aeruginosa and its pyocyanin secretion as a model and an internal standard for self-calibration of SERS signals, the sticky note achieves reliable quantification and a rapid response to the secretion as early as 1 h biofilm growth. With antibiotic loading, the multifunctional SERS sticky note also demonstrated effective inactivation of the bacterial biofilm with simultaneous evaluation of the inactivation effect. This multifunctional SERS sticky note provides a versatile platform for investigating bacterial behaviors and developing antibacterial therapeutics.

中文翻译：

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用于实时群体感应追踪和细菌生物膜失活的多功能SERS便笺†

群体感应（QS）对于细菌的存活和活动至关重要。尽管检测相关的信号代谢物可以揭示QS，但仍非常需要一种多功能平台，用于在细菌生物膜的情况下方便实时地对其分泌物进行实时成像，并抑制生物膜的生长。在这里，我们通过将金纳米星封装在两片六边形氮化硼层之间来开发具有三明治结构的柔性便签，可以轻松地将其粘贴在天然生物膜上，以高灵敏度和时空的SERS成像实时监测分泌的信号分子。解析度。使用铜绿假单胞菌粘滞便笺具有Syo信号的自校准模型和内标，即其紫绿素分泌，可在生物膜生长1 h时实现可靠的定量和对分泌物的快速反应。使用抗生素时，多功能SERS粘滞便笺还显示了细菌生物膜的有效灭活，同时还评估了灭活效果。这款多功能SERS便签纸为调查细菌行为和开发抗菌疗法提供了一个多功能平台。