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Bioinspired multilayer membranes as potential adhesive patches for skin wound healing†
Biomaterials Science ( IF 6.6 ) Pub Date : 2018-05-31 00:00:00 , DOI: 10.1039/c8bm00319j Maria P Sousa 1 , Ana I Neto , Tiago R Correia , Sónia P Miguel , Michiya Matsusaki , Ilídio J Correia , João F Mano
Biomaterials Science ( IF 6.6 ) Pub Date : 2018-05-31 00:00:00 , DOI: 10.1039/c8bm00319j Maria P Sousa 1 , Ana I Neto , Tiago R Correia , Sónia P Miguel , Michiya Matsusaki , Ilídio J Correia , João F Mano
Affiliation
Bioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) and hyaluronic acid modified with dopamine (HA-DN). Freestanding multilayer membranes without DN are also produced as a control. The success of the synthesis of HA-DN was confirmed using UV-visible spectroscopy. Scanning electron microscopy images indicate that the surface of the DN-containing membranes is more porous than the control ones; they also present a higher average thickness value for the same number of CHT/ALG/CHT/HA(-DN) tetralayers (n = 100). Also, water uptake, mechanical strength and adhesion are enhanced with the introduction of DN moieties along the nano-layers. Besides, human dermal fibroblast viability, enhanced adhesion and proliferation were confirmed by immunofluorescence assays and by measuring both the metabolic activity and DNA content. Moreover, in vivo assays with such kinds of DN-containing multilayer membranes were performed; the application of these membranes in the treatment of dermal wounds induced in Wistar rats results in the highest decrease of inflammation of rat skin, compared with the control conditions. Overall, this investigation suggests that these mussel-inspired freestanding multilayer membranes may enhance either their mechanical performance or cellular adhesion and proliferation, leading to an improved wound healing process, being a promising material to restore the structural and functional properties of wounded skin.
中文翻译:
仿生多层膜作为皮肤伤口愈合的潜在粘合贴片†
仿生粘合多层膜是使用壳聚糖 (CHT)、海藻酸盐 (ALG) 和多巴胺改性透明质酸 (HA-DN) 的逐层 (LbL) 组装而成。还生产了不含 DN 的独立式多层膜作为对照。使用紫外-可见光谱证实HA-DN的合成成功。扫描电子显微镜图像表明,含 DN 的膜的表面比对照膜的多孔性更多;对于相同数量的 CHT/ALG/CHT/HA(-DN) 四层 ( n = 100),它们还呈现出更高的平均厚度值。此外,沿着纳米层引入 DN 部分,吸水性、机械强度和粘附力也得到增强。此外,通过免疫荧光测定以及代谢活性和 DNA 含量的测量,证实了人真皮成纤维细胞的活力、增强的粘附和增殖。此外,还对此类含 DN 的多层膜进行了体内测定;与对照条件相比,将这些膜应用于治疗 Wistar 大鼠引起的真皮伤口,可最大程度地减少大鼠皮肤的炎症。总体而言,这项研究表明,这些受贻贝启发的独立式多层膜可以增强其机械性能或细胞粘附和增殖,从而改善伤口愈合过程,成为恢复受伤皮肤的结构和功能特性的有前途的材料。
更新日期:2018-05-31
中文翻译:
仿生多层膜作为皮肤伤口愈合的潜在粘合贴片†
仿生粘合多层膜是使用壳聚糖 (CHT)、海藻酸盐 (ALG) 和多巴胺改性透明质酸 (HA-DN) 的逐层 (LbL) 组装而成。还生产了不含 DN 的独立式多层膜作为对照。使用紫外-可见光谱证实HA-DN的合成成功。扫描电子显微镜图像表明,含 DN 的膜的表面比对照膜的多孔性更多;对于相同数量的 CHT/ALG/CHT/HA(-DN) 四层 ( n = 100),它们还呈现出更高的平均厚度值。此外,沿着纳米层引入 DN 部分,吸水性、机械强度和粘附力也得到增强。此外,通过免疫荧光测定以及代谢活性和 DNA 含量的测量,证实了人真皮成纤维细胞的活力、增强的粘附和增殖。此外,还对此类含 DN 的多层膜进行了体内测定;与对照条件相比,将这些膜应用于治疗 Wistar 大鼠引起的真皮伤口,可最大程度地减少大鼠皮肤的炎症。总体而言,这项研究表明,这些受贻贝启发的独立式多层膜可以增强其机械性能或细胞粘附和增殖,从而改善伤口愈合过程,成为恢复受伤皮肤的结构和功能特性的有前途的材料。