The successful integration of sample pretreatment stages, sensors, actuators and electronics in microfluidic devices enables the attainment of complete micro total analysis systems, also known as lab-on-a-chip devices. In this work, we present a novel monolithic autonomous microanalyzer that integrates microfluidics, electronics, a highly sensitive photometric detection system and a sample pretreatment stage consisting on an embedded microcolumn, all in the same device, for on-line determination of relevant environmental parameters. The microcolumn can be filled/emptied with any resin or powder substrate whenever required, paving the way for its application to several analytical processes: separation, pre-concentration or ionic-exchange. To promote its autonomous operation, avoiding issues caused by bubbles in photometric detection systems, an efficient monolithic bubble removal structure was also integrated. To demonstrate its feasibility, the microanalyzer was successfully used to determine nitrate and nitrite in continuous flow conditions, providing real time and continuous information.

中文翻译：

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高度集成的自主芯片实验室设备，用于在线和就地确定环境化学参数

将样品预处理台，传感器，执行器和电子设备成功集成到微流控设备中，可以实现完整的微量总分析系统，也称为芯片实验室设备。在这项工作中，我们提出了一种新颖的整体式自主微分析仪，该微分析仪将微流体，电子器件，高度灵敏的光度检测系统和由嵌入式微柱组成的样品预处理阶段集成在同一设备中，以在线确定相关的环境参数。只要需要，微柱就可以用任何树脂或粉末基质填充/排空，从而为将其应用于以下分析过程铺平了道路：分离，预浓缩或离子交换。为了促进其自主运行，避免了光度检测系统中气泡引起的问题，还集成了高效的整体气泡去除结构。为了证明其可行性，该微型分析仪已成功用于连续流动条件下的硝酸盐和亚硝酸盐测定，提供了实时和连续的信息。