Five previously undescribed phenylethyl flavones, together with six known flavones, were isolated from the whole plant of Patrinia villosa. It is the first report of natural flavones bearing phenylethyl groups. Their structures were elucidated by the interpretation of 1D and 2D NMR data. The absolute configurations of the previously undescribed phenylethyl flavones were deduced by analysis of the ECD data. All isolates were investigated for their cytoprotective effects against hydrogen peroxide (H2O2)-induced oxidative damage in Caco2 cells for the first time. Moreover, the mRNA expression levels of heme oxygenase-1 (HO-1) and NAD(P)H:quinine oxidoreductase 1 (NQO1), and the expression for mir-144-3p/Nrf2 pathway in Caco2 cells were examined by RT-qPCR analysis. As a result, compound 5 showed most potent cytoprotective activity via modulating mir-144-3p/Nrf2 pathway and thereby scavenging of intracellular ROS in vitro.

中文翻译：

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从绒毛败酱中分离出的未描述的苯乙基黄酮通过调节 mir-144-3p/Nrf2 通路显示出细胞保护特性

五种以前未描述的苯乙基黄酮，连同六种已知的黄酮，是从绿豆败酱全株中分离出来的。这是首次报道带有苯乙基的天然黄酮。通过对 1D 和 2D NMR 数据的解释阐明了它们的结构。先前未描述的苯乙基黄酮的绝对构型是通过分析 ECD 数据推导出来的。首次研究了所有分离株对过氧化氢 (H2O2) 诱导的 Caco2 细胞氧化损伤的细胞保护作用。此外，通过RT-检测血红素加氧酶-1 (HO-1) 和 NAD(P)H:奎宁氧化还原酶 1 (NQO1) 的 mRNA 表达水平，以及 Caco2 细胞中 mir-144-3p/Nrf2 通路的表达。 qPCR 分析。因此，