当前位置: X-MOL 学术Aquat. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Dose- and age-specific antioxidant responses of the mysid crustacean Neomysis awatschensis to metal exposure
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2018-05-28 , DOI: 10.1016/j.aquatox.2018.05.023
Md. Niamul Haque , Do-Hee Lee , Bo-Mi Kim , Sang-Eun Nam , Jae-Sung Rhee

Waterborne metals can adversely affect an organism’s innate defenses through oxidative stress. In the present study, the marine mysid Neomysis awatchensis was exposed to sublethal concentrations (1/10 and 1/5 of the median LC50s) of As, Cd, Cu, Pb, and Zn for 48 or 96 h at the juvenile and adult developmental stages, and the dose- and age-specific antioxidant defense system responses were characterized. Metal accumulation and modulation of four key antioxidant biomarkers, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase, and catalase, were measured in juvenile and adult mysids. Based on LC50, level of metal toxicity was dependent on metal concentration, developmental stage, and the exposure duration. Intracellular MDA content was increased in the As-, Cu-, Pb-, and Zn-exposed juvenile mysids after exposure for 48 and/or 96 h, while increases in MDA content were observed in adult mysids following Cu and Zn exposure. Interestingly, GSH content was differentially modulated, where intracellular GSH levels decreased in juvenile mysids following Cd, Cu, Pb, and Zn exposure, but significantly increased in metal-exposed adult mysids, except for Pb exposure. The catalase and superoxide dismutase activities displayed similar stage-specific increases or decreases as also observed for the different GSH levels, suggesting that the susceptibility to and defense against metal-induced oxidative stress differed based on stage. Modulations in MDA and GSH content and enzymatic activity of the antioxidant defense system indicate that mysid antioxidant defense system factors are intimately connected during control of oxidative imbalances with different capacities at different developmental stages.



中文翻译:

甲壳类甲壳动物Neomysis awatschensis的剂量和年龄特异性抗氧化剂对金属暴露的响应

水性金属可通过氧化应激对有机体的先天防御产生不利影响。在本研究中,海洋mysid awatchensis在青少年和成年发育阶段暴露于亚致死浓度(As,Cd,Cu,Pb和Zn的亚致死浓度(LC50中值的1/10和1/5),分别为48小时或96小时,以及剂量和年龄特异性表征抗氧化防御系统的反应。在幼年和成年类麻疹中测量了四个关键抗氧化剂生物标志物丙二醛(MDA),谷胱甘肽(GSH),超氧化物歧化酶和过氧化氢酶的金属积累和调节。根据LC50,金属毒性水平取决于金属浓度,发育阶段和暴露时间。暴露48、96小时后,暴露于As,Cu,Pb和Zn的青少年类小s中细胞内MDA含量增加,而暴露于Cu和Zn的成年类小s中MDA含量增加。有趣的是,谷胱甘肽含量受到了不同的调节 暴露于Cd,Cu,Pb和Zn的幼年类鱼体中细胞内GSH含量降低,但暴露于金属的成年类鱼体中Pb暴露量显着增加。过氧化氢酶和超氧化物歧化酶活性显示出相似的阶段特异性增加或减少,也针对不同的GSH含量观察到,表明对金属诱导的氧化应激的敏感性和防御能力因阶段而异。MDA和GSH含量以及抗氧化防御系统酶活性的调节表明,在不同发育阶段,具有不同能力的氧化失衡控制过程中,mysid抗氧化防御系统因子紧密相关。但是,除了铅暴露外,成年人的金属暴露的成人类mysids显着增加。过氧化氢酶和超氧化物歧化酶活性显示出相似的阶段特异性增加或减少,也针对不同的GSH含量观察到,表明对金属诱导的氧化应激的敏感性和防御能力因阶段而异。MDA和GSH含量以及抗氧化防御系统酶活性的调节表明,在不同发育阶段,具有不同能力的氧化失衡控制过程中,mysid抗氧化防御系统因子紧密相关。但是,除了铅暴露外,成年人的金属暴露的成人类mysids显着增加。过氧化氢酶和超氧化物歧化酶活性显示出相似的阶段特异性增加或减少,也针对不同的GSH含量观察到,表明对金属诱导的氧化应激的敏感性和防御能力因阶段而异。MDA和GSH含量以及抗氧化防御系统酶活性的调节表明,在不同发育阶段,具有不同能力的氧化失衡控制过程中,mysid抗氧化防御系统因子紧密相关。这表明对金属诱导的氧化应激的敏感性和防御能力因阶段而异。MDA和GSH含量以及抗氧化防御系统酶活性的调节表明,在不同发育阶段,具有不同能力的氧化失衡控制过程中,mysid抗氧化防御系统因子紧密相关。这表明对金属诱导的氧化应激的敏感性和防御能力因阶段而异。丙二醛和谷胱甘肽含量和抗氧化防御系统酶活性的调节表明,在不同发育阶段,具有不同能力的氧化失衡控制过程中,mysid抗氧化防御系统因子紧密相关。

更新日期:2018-05-28
down
wechat
bug