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The divide and conquer strategies for deep phosphoproteomics analysis
Trends in Analytical Chemistry ( IF 13.1 ) Pub Date : 2018-05-30 , DOI: 10.1016/j.trac.2018.05.015
Mingming Dong , Yating Yao , Yan Wang , Yan Jin , Mingliang Ye

With the advance of mass spectrometry based proteomics techniques, thousands of phosphorylation sites can be identified from a single cell line or a single tissue sample by large scale global analysis. However, such large scale analysis is time consuming. And, due to the high complexity of the phosphoproteome sample, this approach is often unable to identify low abundance regulatory phosphorylation sites. Alternatively, the “divide and conquer” strategy by targeted analysis of a subset of phosphoproteome, i.e. subphosphoproteome, can improve the detection sensitivity of these phosphorylation sites. In this review, we summarized the analytical methods in this field. The enrichment approaches for the analysis of subphosphoproteomes generated by tyrosine kinases and basophilic kinases were introduced. The strategies for the analysis of the subphosphoproteomes composed by endogenous phosphopeptides, multiply phosphorylated peptides, etc., were also reviewed.



中文翻译:

深度磷酸化蛋白质组学分析的分而治之策略

随着基于质谱的蛋白质组学技术的发展,可以通过大规模全局分析从单个细胞系或单个组织样品中鉴定出数千个磷酸化位点。但是,这样的大规模分析非常耗时。而且,由于磷酸化蛋白质组样品的高度复杂性,这种方法通常无法识别低丰度的调节性磷酸化位点。或者,通过对磷酸化蛋白质组的一部分(即亚磷酸化蛋白质组)进行有针对性的分析,“分而治之”的策略可以提高这些磷酸化位点的检测灵敏度。在这篇综述中,我们总结了该领域的分析方法。酪氨酸产生的亚磷酸化蛋白质组分析的富集方法引入了激酶和嗜碱性激酶。还回顾了分析由内源性磷酸肽,多重磷酸化肽等组成的亚磷酸化蛋白质组的策略。

更新日期:2018-07-12
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