The laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymerization using US or HPH. Molecular dynamic simulation studies supported these findings by revealing a more open enzyme active site upon environments with high molecular agitation. The higher mass transport generated by US and HPH is the main feature responsible for a higher substrate accessibility to the enzyme which contributed to produce longer polymers.

使用不同的反应器，即水浴（WB），超声浴（US）和高压均化器（HPH），进行邻苯二酚的漆酶聚合。游离OH的总含量和所获得聚合物的MALDI-TOF光谱表明，在高能环境下，反应易于进行。使用US或HPH进行聚合后，可获得更高的转化率和聚合度（DP）。分子动力学模拟研究通过揭示在高分子搅动环境下更开放的酶活性位点来支持这些发现。US和HPH产生的较高质量的运输是该酶具有较高的底物可及性的主要特征，该酶有助于产生更长的聚合物。