Small berry fruit products are gaining an expanded market due to their high nutrition value. However, the authenticity of products is challenged by adulteration and mislabeling. To establish an accurate and robust method for identifying both known and unknown fruit species in small berry fruit products, DNA barcoding technology based on Sanger sequencing was adopted. To overcome the influence of processing conditions on DNA recovery, mini-barcodes of rbcL and ITS and a medium-barcode of psbA-trnH were applied. To identify ingredients in products containing mixed species, plasmid cloning was applied to separate mixed barcodes. The method established in this paper could detect 1% to 10% target species in mixed fruit juice.

中文翻译：

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DNA条形码法鉴定水果产品中的小浆果

由于其高营养价值，小浆果产品正在扩大市场。然而，产品的真实性受到掺假和标签错误的挑战。为了建立一种准确可靠的方法来识别小浆果产品中已知和未知的水果种类，采用了基于 Sanger 测序的 DNA 条形码技术。为了克服处理条件对 DNA 回收的影响，使用了 rbcL 和 ITS 的迷你条形码以及 psbA-trnH 的中等条形码。为了识别包含混合物种的产品中的成分，应用质粒克隆来分离混合条形码。本文建立的方法可以检测混合果汁中 1% 到 10% 的目标物种。