In this study, an antimicrobial platform in the form of nitric oxide (NO) gas‐releasing polydopamine (PDA)‐coated iron oxide nanoparticles (IONPs) is developed for combating bacterial biofilms. NO is bound to the PDA‐coated IONPs via the reaction between NO and the secondary amine moieties on PDA to form N‐diazeniumdiolate (NONOate) functionality. To impart colloidal stability to the nanoparticles in aqueous solutions (e.g., phosphate buffered saline (PBS) and bacteria cell culture media M9), a polymer bearing hydrophilic and amine pendant groups, P(OEGMA)‐b‐P(ABA), is synthesized via reversible addition‐fragmentation chain transfer (RAFT) polymerization and is subsequently grafted onto the PDA‐coated IONPs by employing the Schiff base/Michael addition reaction between o‐quinone and a primary amine. These nanoparticles are able to effectively disperse Pseudomonas aeruginosa biofilms (up to 79% dispersal) at submicromolar NO concentrations. In addition, the nanoparticles demonstrate excellent bactericidal activity toward P. aeruginosa planktonic and biofilm cells (up to 5‐log₁₀ reduction).

中文翻译：

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利用聚多巴胺涂层纳米颗粒的多功能性来提供一氧化氮和防治细菌生物膜。

在本研究中，开发了一种以释放一氧化氮（NO）的聚多巴胺（PDA）涂层的氧化铁纳米颗粒（IONPs）形式的抗菌平台，用于对抗细菌生物膜。通过NO和PDA上仲胺部分之间的反应，NO结合到PDA包覆的IONP上，形成N-重氮二醇二酸酯（NONOate）官能团。为了赋予纳米颗粒在水溶液中的胶体稳定性（例如，磷酸盐缓冲盐水（PBS）和细菌细胞培养基M9），合成了带有亲水和胺侧基的聚合物P（OEGMA）-b- P（ABA）通过可逆的加成-断裂链转移（RAFT）聚合反应，随后通过邻位之间的席夫碱/迈克尔加成反应将其接枝到PDA包覆的IONP上醌和伯胺。这些纳米颗粒能够以亚微摩尔NO浓度有效分散铜绿假单胞菌生物膜（分散度高达79％）。此外，纳米颗粒对铜绿假单胞菌的浮游生物和生物膜细胞显示出优异的杀菌活性（减少了5-log ₁₀）。