Abstract Development of new methods for rapid and effective detection of hydrogen sulfite is of great importance in the studies of this species in food samples. In this contribution, a ruthenium(II) complex-based luminescence probe, Ru-CHO , was developed for fast, highly sensitive and selective recognition and detection of hydrogen sulfite. Ru-CHO was found to be weakly luminescent due to the intramolecular charge transfer dominated by the strong electron withdrawing aldehyde groups. In the presence of hydrogen sulfite, the aldehyde groups in Ru-CHO can specifically react with hydrogen sulfite, which results in the formation of a new ruthenium(II) complex Ru-SO , to lead to the remarkable luminescence enhancement. The recognition reaction was found to be completed in a few seconds, and the dose-dependent increase in luminescence intensity showed good linearity against the hydrogen sulfite concentration, suggested the availability of Ru-CHO for the rapid quantification of hydrogen sulfite in aqueous media. Hydrogen sulfite promoted reaction of Ru-CHO was confirmed by ESI-MS and 1 H NMR analysis, and the photophysical properties, luminescence changes and the electronic transitions in Ru-CHO and Ru-SO were validated by density functional theory (DFT)/time-dependent DFT (TDDFT) calculations. Ru-CHO was successfully used for the quantitative detection of hydrogen sulfite in wine and sugar samples , which validated the practical applicability of Ru-CHO as a luminescence probe for the detection of hydrogen sulfite in food samples.

中文翻译：

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开发用于检测食品样品中亚硫酸氢盐的基于钌（II）配合物的发光探针

摘要 开发快速有效检测亚硫酸氢的新方法对于食品样品中该物种的研究具有重要意义。在这项贡献中，开发了一种基于钌 (II) 配合物的发光探针 Ru-CHO ，用于快速、高灵敏度和选择性地识别和检测亚硫酸氢盐。由于强吸电子醛基主导的分子内电荷转移，Ru-CHO 被发现是微弱的发光。在亚硫酸氢盐存在下，Ru-CHO中的醛基可以与亚硫酸氢盐发生特异性反应，形成新的钌（II）络合物Ru-SO ，从而导致显着的发光增强。发现识别反应在几秒钟内完成，发光强度的剂量依赖性增加与亚硫酸氢浓度呈良好的线性关系，表明 Ru-CHO 可用于快速定量水介质中的亚硫酸氢盐。通过 ESI-MS 和 1 H NMR 分析证实亚硫酸氢促进 Ru-CHO 反应，并通过密度泛函理论 (DFT)/时间验证 Ru-CHO 和 Ru-SO 的光物理性质、发光变化和电子跃迁依赖 DFT (TDDFT) 计算。Ru-CHO成功用于葡萄酒和糖类样品中亚硫酸氢盐的定量检测，验证了Ru-CHO作为发光探针检测食品样品中亚硫酸氢盐的实用性。建议使用 Ru-CHO 快速定量水介质中的亚硫酸氢盐。通过 ESI-MS 和 1 H NMR 分析证实亚硫酸氢促进 Ru-CHO 反应，并通过密度泛函理论 (DFT)/时间验证 Ru-CHO 和 Ru-SO 的光物理性质、发光变化和电子跃迁依赖 DFT (TDDFT) 计算。Ru-CHO成功用于葡萄酒和糖类样品中亚硫酸氢盐的定量检测，验证了Ru-CHO作为发光探针检测食品样品中亚硫酸氢盐的实用性。建议使用 Ru-CHO 快速定量水介质中的亚硫酸氢盐。通过 ESI-MS 和 1 H NMR 分析证实亚硫酸氢促进 Ru-CHO 反应，并通过密度泛函理论 (DFT)/时间验证 Ru-CHO 和 Ru-SO 的光物理性质、发光变化和电子跃迁依赖 DFT (TDDFT) 计算。Ru-CHO成功用于葡萄酒和糖类样品中亚硫酸氢盐的定量检测，验证了Ru-CHO作为发光探针检测食品样品中亚硫酸氢盐的实用性。Ru-CHO 和 Ru-SO 中的发光变化和电子跃迁通过密度泛函理论 (DFT)/时间相关 DFT (TDDFT) 计算得到验证。Ru-CHO成功用于葡萄酒和糖类样品中亚硫酸氢盐的定量检测，验证了Ru-CHO作为发光探针检测食品样品中亚硫酸氢盐的实用性。Ru-CHO 和 Ru-SO 中的发光变化和电子跃迁通过密度泛函理论 (DFT)/时间相关 DFT (TDDFT) 计算得到验证。Ru-CHO成功用于葡萄酒和糖类样品中亚硫酸氢盐的定量检测，验证了Ru-CHO作为发光探针检测食品样品中亚硫酸氢盐的实用性。