Abstract A comprehensive picture of the chemical composition and structure of the acyl-glycerides from ergot (fungi of the genus Claviceps) sclerotia was carried out using liquid chromatography coupled with high resolution mass spectrometry (HPLC-ESI-Q-ToF) following microwave solvent extraction. The method allowed us to study the chemical structures of >70 compounds being most of these reported and characterized here for the first time in ergot sclerotia. Diglycerides, triglycerides and estolides were proper preliminary separated using reverse phase chromatography and the unambiguous chemical structure of each species was characterized using high resolution tandem mass spectrometry. Our data and results could be useful for anyone interested in detecting ergot contaminations in cereals and deriving products, as alternative to the identification of alkaloids, in better understanding the behavior of ergot fungi in biological contest and in evaluating the potential uses of ergot estolides in industrial applications.

中文翻译：

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高效液相色谱与高分辨率质谱联用对麦角酰基甘油酯化学结构的研究进展

摘要 使用液相色谱-高分辨质谱联用 (HPLC-ESI-Q-ToF) 在微波溶剂萃取后，对麦角（Claviceps 属真菌）菌核中的酰基甘油酯的化学成分和结构进行了全面分析。 . 该方法使我们能够研究 > 70 种化合物的化学结构，这些化合物中的大部分是首次在麦角菌核中报告和表征的化合物。使用反相色谱对甘油二酯、甘油三酯和 estolides 进行适当的初步分离，并使用高分辨率串联质谱法表征每个物种的明确化学结构。我们的数据和结果可能对任何有兴趣检测谷物和衍生产品中的麦角污染的人有用，