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Mixture design and Doehlert matrix for optimization of the ultrasonic assisted extraction of caffeic acid, rutin, catechin and trans -cinnamic acid in Physalis angulata L. and determination by HPLC DAD
Microchemical Journal ( IF 4.8 ) Pub Date : 2018-09-01 , DOI: 10.1016/j.microc.2018.04.035
Gisele Chagas Moreira , Fabio de Souza Dias

Abstract The present article describes the development of an ultrasonic assisted extraction of phenolic compounds in Physalis angulata and determination of caffeic acid, rutin, catechin and trans-cinnamic acid by HPLC DAD. The mixtures were composed of extractor solvent and its ratio optimized using a mixture design. Doehlert matrix was used to optimize the extractor solvent volume and sonication time while using total phenolics as analytical response. The optimized conditions for the extractor solvent were the ratio of: 57% water, 35% ethanol and 8% methanol. The best experimental conditions for ultrasound were a sonication time of 10 min and extractor volume of 15 mL. The limit of detection varied from 5.0 to 30 μg L−1. The methods precision was expressed as relative standard deviation (RSD) and presented values lower than 3.0% for all analytes. The accuracy was evaluated using spike test with recoveries between 91% and 107%. The method was applied for the determination of caffeic acid, rutin, catechin and trans-cinnamic in four samples of Physalis angulata aerial parts and the concentration varied from 15.5 to 22.5 μg g−1 for catechin, from 25.0 to 32.5 μg g−1 for caffeic acid, 75.6 to 88.2 μg g−1 for rutin and varied from 23.5 to 110.7 μg g−1 for trans-cinnamic acid.

中文翻译:

混合物设计和 Doehlert 矩阵用于优化超声辅助提取 Physalis angulata L. 中咖啡酸、芦丁、儿茶素和反式肉桂酸并通过 HPLC DAD 测定

摘要 本文描述了酸浆中酚类化合物的超声波辅助提取方法的开发,以及用 HPLC DAD 测定咖啡酸、芦丁、儿茶素和反式肉桂酸的方法。混合物由萃取溶剂组成,并使用混合物设计优化其比例。Doehlert 矩阵用于优化萃取溶剂体积和超声处理时间,同时使用总酚作为分析响应。萃取溶剂的优化条件为:57%的水、35%的乙醇和8%的甲醇。超声的最佳实验条件是超声处理时间为 10 分钟,提取器体积为 15 毫升。检测限从 5.0 到 30 μg L-1 不等。方法精密度表示为相对标准偏差 (RSD),所有分析物的值均低于 3.0%。使用加标测试评估准确度,回收率在 91% 到 107% 之间。该方法用于测定4个酸浆地上部分样品中的咖啡酸、芦丁、儿茶素和反式肉桂酸,儿茶素浓度为15.5~22.5 μg g−1,儿茶素浓度为25.0~32.5 μg g−1。咖啡酸,芦丁为 75.6 至 88.2 μg g-1,反式肉桂酸为 23.5 至 110.7 μg g-1。
更新日期:2018-09-01
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