In the present study, we evaluate the N₂⁺/OH emission intensity ratio as a diagnostic tool for identifying the best instrumental operating conditions in microwave-induced plasma optical emission spectrometry (MIP OES). This molecular species signal ratio is compared with the traditional Mg II/Mg I ratio. Aluminum, Ba, Mn, Sr and Zn (analytes), and high concentrations of C, Na, Ca, HNO₃ and HCl (sample matrices) are used as models to investigate the effects of complex matrices on analyte recoveries. The N₂⁺/OH signal ratio is more sensitive to changes in plasma conditions than the Mg II/Mg I ratio. Some other advantages include real-time monitoring capabilities, and the possibility of independently tracking variations in both plasma and sample introduction. For less complex matrices, the N₂⁺/OH signal ratio may be used for instrument optimization, which ensures plasma conditions are as similar as possible when analyzing standard solutions and samples. For analyses involving severe matrix effects, molecular species such as CN, N₂, N₂⁺ and OH are used for signal correction. Significant improvements in accuracy are achieved by employing the analyte-to-molecular species signal ratio, or their product, for calibration. Both the use of the N₂⁺/OH signal ratio as a diagnostic tool, and of molecular species for signal correction to minimize matrix effects are simple strategies that may significantly contribute to expanding the analytical capabilities of MIP OES and facilitating its application in routine analysis.

中文翻译：

---

天然存在的分子种类，用于微波诱导的等离子体光发射光谱法中的等离子体诊断和信号校正†

在本研究中，我们评估N ₂ ⁺ / OH发射强度比，作为在微波诱导等离子体发射光谱（MIP OES）中识别最佳仪器操作条件的诊断工具。将该分子种类的信号比率与传统的Mg II / Mg I比率进行比较。铝，钡，锰，锶和锌（分析物）以及高浓度的碳，钠，钙，HNO ₃和HCl（样品基质）被用作模型，以研究复杂基质对分析物回收率的影响。N ₂ ⁺/ OH信号比对血浆条件的变化比Mg II / Mg I比更敏感。其他一些优势包括实时监控功能，以及独立跟踪血浆和样品引入变化的可能性。对于不太复杂的基质，可以将N ₂ ⁺ / OH信号比用于仪器优化，以确保在分析标准溶液和样品时血浆条件尽可能相似。对于涉及严重基质效应的分析，将分子种类（例如CN，N ₂，N ₂ ⁺和OH）用于信号校正。通过使用分析物与分子种类的信号比率或其乘积进行校准，可实现准确性的显着提高。两者同时使用₂ ⁺ / OH信号比作为诊断工具，以及用于信号校正的分子种类以最小化基质效应是简单的策略，可能会极大地扩展MIP OES的分析能力并促进其在常规分析中的应用。