High-fat diet (HFD) alters gut microbial diversity and drives a rapid development of hypothalamic inflammation, which impairs hypothalamic insulin sensitivity. The addition of berries in the diet may promote beneficial effects in the host. We investigated the potential of juçara with different doses on the reshaping of the microbiota and improvement in the hypothalamic insulin resistance (HIR) induced by acute HFD treatment. We analyzed pro and anti-inflammatory protein content and expression by ELISA (blood and colon) and Western blotting (hypothalamus); gene expression by RT-PCR (colon and stool). Juçara supplementation restored the fecal Bifidobacteria content, reduced colon inflammation and minimized HIR. It also down-regulated IRα and up-regulated pAkt and increased pAkt/Akt ratio. The lower dose was protective against the adverse effects of HFD, by enhancing IRα efficiency in the hypothalamus. To our knowledge, this is the first study to elucidate the role of juçara in the HIR.

高脂饮食（HFD）会改变肠道微生物的多样性，并导致下丘脑炎症的快速发展，从而损害下丘脑的胰岛素敏感性。在饮食中添加浆果可以促进宿主的有益作用。我们调查了不同剂量jujuara对微生物群的重塑和急性HFD治疗诱导的下丘脑胰岛素抵抗（HIR）改善的潜力。我们通过ELISA（血液和结肠）和蛋白质印迹（下丘脑）分析了促炎蛋白和抗炎蛋白的含量和表达；通过RT-PCR进行基因表达（冒号和粪便）。Juçara补充剂可恢复粪便中双歧杆菌的含量，减少结肠炎症并使HIR降至最低。它还下调了IRα，上调了pAkt，并增加了pAkt / Akt比。较低剂量可通过增强下丘脑中的IRα效率来保护人体免受HFD的不利影响。据我们所知，这是第一个阐明juçara在HIR中的作用的研究。