Chlorpyrifos (CP), an organophosphate insecticide is broadly used in the agricultural and industrial sectors to control a broad-spectrum of insects of economically important crops. CP detection has been gaining prominence due to its widespread contamination in different environmental matrices, high acute toxicity, and potential to cause long-term environmental and ecological damage even at trace levels. Traditional chromatographic methods for CP detection are complex and require sample preparation and highly skilled personnel for their operation. Over the past decades, electrochemical biosensors have emerged as a promising technology for CP detection as these circumvent deficiencies associated with classical chromatographic techniques. The advantageous features such as appreciable detection limit, miniaturization, sensitivity, low-cost and onsite detection potential are the propulsive force towards sustainable growth of electrochemical biosensing platforms. Recent development in enzyme immobilization methods, novel surface modifications, nanotechnology and fabrication techniques signify a foremost possibility for the design of electrochemical biosensing platforms with improved sensitivity and selectivity. The prime objective of this review is to accentuate the recent advances in the design of biosensing platforms based on diverse biomolecules and biomimetic molecules with unique properties, which would potentially fascinate their applicability for detection of CP residues in real samples. The review also covers the sensing principle of the prime biomolecule and biomimetic molecule based electrochemical biosensors along with their analytical performance, advantages and shortcomings. Present challenges and future outlooks in the field of electrochemical biosensors based CP detection are also discussed. This deep analysis of electrochemical biosensors will provide research directions for further approaching towards commercial development of the broad range of organophosphorus compounds.

毒死rif（CP）是一种有机磷酸盐杀虫剂，广泛用于农业和工业部门，以控制具有重要经济意义的农作物的广谱昆虫。CP检测由于其在不同环境基质中的广泛污染，高急性毒性以及即使在痕量水平下也可能造成长期的环境和生态破坏的潜力而日益受到关注。用于CP检测的传统色谱方法很复杂，需要样品制备和高技能的操作人员。在过去的几十年中，电化学生物传感器已成为一种有前途的CP检测技术，因为这些规避了传统色谱技术带来的缺陷。有利的功能，例如可观的检测极限，小型化，灵敏度高，低成本和现场检测的潜力是电化学生物传感平台可持续发展的推动力。酶固定方法，新型表面修饰，纳米技术和制造技术的最新发展标志着设计具有更高灵敏度和选择性的电化学生物传感平台的最大可能性。这篇综述的主要目的是强调基于具有独特特性的各种生物分子和仿生分子的生物传感平台设计的最新进展，这可能会吸引它们在实际样品中检测CP残留物的适用性。审查还涵盖了基于主要生物分子和仿生分子的电化学生物传感器的传感原理及其分析性能，优点和缺点。还讨论了基于CP检测的电化学生物传感器领域中的当前挑战和未来前景。对电化学生物传感器的深入分析将为进一步发展广泛的有机磷化合物的商业开发提供研究方向。