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Electrochemical immunoassay for the detection of IgM antibodies using polydopamine particles loaded with PbS quantum dots as labels
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2018-05-26 , DOI: 10.1016/j.bios.2018.05.046
Greter A. Ortega , Julio C. Zuaznabar-Gardona , Edilso Reguera

Here, we report for the first time, an electrochemical immunoassay to detect IgM antibodies using lead sulfide quantum dots (PbS QDs) as electrochemical labels. In this sense, dendritic-like polydopamine particles loaded with PbS QDs were synthesized by the self-polymerization of dopamine in basic media in the presence of QDs ([email protected]) and further tagged with anti-IgM antibodies, dengue specific antigens, and streptavidin moieties. The analytical features of the sandwich immunoassay on ELISA microplate were carried out with the [email protected] anti-IgM as secondary antibody. The system was interrogated by acid dissolution of [email protected], followed by differential pulse anodic stripping voltammetry in the presence of Bi(III) ions using carbon screen-printed electrodes. The results indicate that the voltammetric current increased with the increasing of the concentration of target IgM within a range of 0–0.5 mg mL−1. The limit of detection of this electrochemical immunoassay was evaluated to 130 ng. The measures of satisfactory recoveries from 88.5% to 114% of spiked samples indicate that such a method has good specificity and is applicable to the quantification of IgM antibodies in complex biological samples. No significant differences at the 0.05 significance level were encountered in the analysis of IgM samples between the electrochemical immunoassay and a Bradford assay.



中文翻译:

电化学免疫法检测IgM抗体,使用负载PbS量子点的聚多巴胺颗粒作为标记

在这里,我们首次报告了使用硫化铅量子点(PbS QDs)作为电化学标记物检测IgM抗体的电化学免疫测定方法。从这个意义上讲,在存在QD的情况下,多巴胺在碱性介质中通过自聚合来合成负载PbS QD的树突状聚多巴胺颗粒([电子邮件保护]),并进一步用抗IgM抗体,登革热特异性抗原和链霉亲和素部分。用[电子邮件保护]抗IgM作为二抗,在ELISA微板上进行了夹心免疫测定的分析特征。通过[电子邮件保护]的酸溶解来询问该系统,然后使用碳丝网印刷电极在Bi(III)离子存在下进行差分脉冲阳极溶出伏安法。-1。该电化学免疫测定的检测极限被评估为130ng。从加标样品的88.5%至114%的满意回收率的测量结果表明,这种方法具有良好的特异性,可用于定量复杂生物样品中的IgM抗体。在电化学免疫测定和Bradford测定之间的IgM样品分析中,在0.05显着性水平上没有发现显着差异。

更新日期:2018-05-26
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