Aflatoxin M1 (AFM1) contamination presents one of the most serious concerns in milk safety. In this study, the immobilization of Saccharomyces cerevisiae was used to detoxify AFM1-contaminated milk. The yeasts were immobilized on perlite for 24 and 48 hr, and the best immobilization time was achieved at 48 hr. Microscopic examination confirmed successful immobilization. The milk samples with 0.08, 0.13, 0.18, and 0.23 ppb AFM1 contamination were passed through the biofilter for 20, 40, and 80 min. The results showed a significant reduction in AFM1 concentration for all the milk samples with various initial AFM1 contents. The contaminated milk with 0.08 ppb AFM1 was completely cleared after 40 min of circulation while for the milk solution with 0.23 ppb, the highest AFM1 reduction was obtained at about 81.3% after 80 min circulation. In addition, the biofilter was saturated after the third step of milk circulation, containing 0.23 ppb AF, in which each step duration was 20 min. This study showed the excellent capability of the immobilized cells on the perlite beads to detoxify the AFM1-contaminated milk without any side effects on its physicochemical properties. PRACTICAL APPLICATION The immobilization of Saccharomyces cerevisiae cells on perlite beads can be used to detoxify AFM1-contaminated milk. The perlite can provide a perfect support for immobilization. With respect to qualitative properties, 20 min, was suggested as the optimum time for milk decontamination. This study indicated that the detoxification of contaminated milk using immobilized S. cerevisiae cells on the perlite support did not affect the different properties of detoxified milk. This method can lead to a practical solution to address aflatoxin contamination in dairy products considered high-risk foods.

中文翻译：

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将酿酒酵母固定在珍珠岩珠上去除牛奶中的黄曲霉毒素 M1

黄曲霉毒素 M1 (AFM1) 污染是牛奶安全中最严重的问题之一。在这项研究中，酿酒酵母的固定化被用于对 AFM1 污染的牛奶进行解毒。酵母在珍珠岩上固定 24 和 48 小时，最佳固定时间为 48 小时。显微镜检查证实成功固定。具有 0.08、0.13、0.18 和 0.23 ppb AFM1 污染的牛奶样品通过生物过滤器 20、40 和 80 分钟。结果表明，具有不同初始 AFM1 含量的所有牛奶样品的 AFM1 浓度显着降低。含有 0.08 ppb AFM1 的污染牛奶在循环 40 分钟后被完全清除，而对于 0.23 ppb 的牛奶溶液，在循环 80 分钟后获得最高的 AFM1 减少量约为 81.3%。此外，生物过滤器在牛奶循环的第三步后饱和，含有 0.23 ppb AF，其中每步持续时间为 20 分钟。这项研究表明，珍珠岩珠上的固定细胞具有出色的能力，可以对 AFM1 污染的牛奶进行解毒，而对其物理化学性质没有任何副作用。实际应用 将酿酒酵母细胞固定在珍珠岩珠上可用于对 AFM1 污染的牛奶进行解毒。珍珠岩可以为固定提供完美的支撑。关于定性特性，建议 20 分钟作为牛奶去污的最佳时间。该研究表明，在珍珠岩载体上使用固定化酿酒酵母细胞对污染牛奶进行解毒不会影响解毒牛奶的不同特性。