Antibacterial agents with two-photon absorption are expected to play a significant role in biomedical science. Herein, two novel organotin complexes, HLSn1 and HLSn2, based on coumarin were designed, synthesized, and systematically investigated. It was found that these complexes possessed suitable two-photon-active cross sections in the near-infrared region. Moreover, complex HLSn1 could efficiently inhibit the growth of Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, especially the latter with a minimum inhibitory concentration (MIC; 90%) of 2 ± 0.14 μg mL^–1, which is lower than that of Kanamycin (Kana, 8 ± 0.42 μg mL^–1). Importantly, two-photon imaging and superresolution development of bacterial stain revealed that complex HLSn1 can react with bacterial membranes, producing reactive oxygen species (ROS) and leading to cell death. These outcomes provide promising applications in the superresolution bacteria imaging, diagnostics, and treatment of bacterial infectious.

中文翻译：

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两光子活性有机锡（IV）配合物的抗菌功能和超分辨率细菌成像。

具有双光子吸收的抗菌剂有望在生物医学领域中发挥重要作用。在此，设计，合成并系统研究了两种基于香豆素的新型有机锡复合物HLSn1和HLSn2。发现这些络合物在近红外区域具有合适的两光子活性截面。此外，复杂的HLSn1可以有效抑制革兰氏阴性大肠杆菌和枯草芽孢杆菌阳性芽孢杆菌的生长，尤其是后者的最低抑菌浓度（MIC; 90％）为2±0.14μgmL ^–1，低于卡那霉素（Kana，8±0.42μgmL ^–1）。重要的是，细菌污渍的双光子成像和超分辨率显影表明，复杂的HLSn1可以与细菌膜反应，产生活性氧（ROS）并导致细胞死亡。这些结果为超分辨率细菌成像，细菌感染的诊断和治疗提供了有希望的应用。