Miniaturization of liquid chromatography (LC) is at the leading edge of the research topics and offers a wide range of applications in chemistry, biology, pharmaceutical, clinical diagnosis, and food analysis. The state-of-the-art “lab-on-chip” or “micro-total analysis systems” gathered scientists all over the world to provide systems with the aim of portability, reliability, reduced analysis time and cost of operation and analysis. From the hardware equipment point of view, the LC system is made by the primary compartments of solvent reservoir, pump, injector, column, and detector. In this work, we surveyed the development of LC systems and advances in each of the integrated components (i.e. pumping systems, injectors, separation channels, and detector) and the materials used for their fabrication from the beginning up to now. Finally, we summarize the application of fully integrated LC systems and exemplify about the multidimensional LCs on a chip development.

液相色谱仪（LC）的微型化是研究主题的前沿，并在化学，生物学，制药，临床诊断和食品分析中提供了广泛的应用。最新的“片上实验室”或“微总量分析系统”聚集了世界各地的科学家，旨在为系统提供可移植性，可靠性，减少分析时间以及降低操作和分析成本的目标。从硬件设备的角度来看，液相色谱系统由溶剂储存器，泵，进样器，色谱柱和检测器的主隔室组成。在这项工作中，我们调查了液相色谱系统的发展以及每个集成组件（即泵系统，喷射器，分离通道和检测器）以及从一开始到现在所用的制造材料。最后，我们总结了完全集成的LC系统的应用，并举例说明了芯片开发中的多维LC。