Zein films added with different concentrations (0, 25, 50, & 75 mg/ml of film forming solution) of pomegranate peel extract (PPE) were designed and characterized as a novel packaging material for Himalayan cheese (kalari). With increase in PPE concentration, tensile strength, elongation at break, total phenolic content and antioxidant activity of zein films increased whereas film solubility, water vapor transmission rate decreased and thickness remained constant. PPE containing films exhibited inhibitory activity against all tested pathogenic strains (Escherchia coli, Pseudomonas perfringens, Micrococcus luteus, Enterococci faecalis, Staphylococcus aureus, Proteus vulgaris and Salmonella typhii). ATR-FTIR spectra showed interactions between PPE polyphenols and zein film. Kalari wrapped in PPE containing zein films were found to have low protein and lipid oxidation products during storage when compared to film without PPE. Films were also found to inhibit spoilage microorganisms in kalari whereas number of Lactic acid bacteria was enhanced. The study validated that incorporation of fruit waste (pomegranate peel in this case) which are rich in polyphenols, in zein film offers a promising source for inducing it the ability to control cheese spoilage caused either by the bacterial contamination or protein and fat oxidation, without affecting its overall sensory characteristics.

设计添加了不同浓度（0、25、50和75 mg / ml的成膜溶液）石榴皮提取物（PPE）的玉米蛋白膜，并将其作为喜马拉雅奶酪（kalari）的新型包装材料进行了表征。随着PPE浓度的增加，玉米蛋白膜的抗张强度，断裂伸长率，总酚含量和抗氧化活性增加，而膜溶解度，水蒸气透过率降低并且厚度保持恒定。含PPE的膜对所有测试的病原菌株（大肠杆菌，产气假单胞菌，黄球菌，粪肠球菌，金黄色葡萄球菌，寻常变形杆菌和变形杆菌）均表现出抑制作用。伤寒沙门氏菌）。ATR-FTIR光谱显示了PPE多酚与玉米醇溶蛋白膜之间的相互作用。与不含PPE的薄膜相比，包裹在含PPE的玉米醇溶蛋白薄膜中的Kalari在储存过程中具有低蛋白质和脂质氧化产物。还发现薄膜抑制了卡拉利腐烂微生物，而乳酸菌的数量增加了。这项研究证实，在玉米蛋白膜中掺入富含多酚的水果废料（在这种情况下为石榴皮）提供了一个有希望的来源，可以诱导其控制由细菌污染或蛋白质和脂肪氧化引起的奶酪变质的能力，而无需影响其整体感官特征。