The chemical biology of reactive sulfur species, including hydropolysulfides, has been a subject undergoing intense study in recent years, but further understanding of their “intact” function in living cells has been limited owing to a lack of appropriate analytical tools. In order to overcome this limitation, we developed a new type of fluorescent probe that reversibly and selectively reacts to hydropolysulfides. The probe enables live‐cell visualization and quantification of endogenous hydropolysulfides without interference from intrinsic thiol species such as glutathione. Additionally, real‐time reversible monitoring of oxidative‐stress‐induced fluctuation of intrinsic hydropolysulfides has been achieved with a temporal resolution on the order of seconds, a result which has not yet been realized using conventional methods. These results reveal the probe's versatility as a new fluorescence imaging tool to understand the function of intracellular hydropolysulfides.

中文翻译：

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可逆荧光探针，用于实时活细胞成像和定量内源性氢硫化物

近年来，包括氢多硫化物在内的反应性硫物种的化学生物学研究一直很深入，但是由于缺乏适当的分析工具，对它们在活细胞中“完整”功能的进一步理解受到了限制。为了克服此限制，我们开发了一种新型的荧光探针，该探针可逆并选择性地与氢硫化物反应。该探针可实现活细胞可视化和内源性氢硫化物定量，而不受内在硫醇物种（如谷胱甘肽）的干扰。此外，已经实现了对氧化应力引起的内在多硫化物波动的实时可逆监测，其时间分辨率约为几秒钟，而使用传统方法尚无法实现这一结果。