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Highly selective aptamer based organic electrochemical biosensor with pico-level detection
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2018-05-22 , DOI: 10.1016/j.bios.2018.05.031
Nileshi Saraf , Eric R. Woods , Madison Peppler , Sudipta Seal

An organic aptamer functionalized electrochemical transistor has been developed to detect the presence of epinephrine molecule which acts as an excitatory neurotransmitter. The abnormalities in the level of epinephrine are the direct symptoms of some diseases such as Takotsubo cardiomyopathy, myocardial infarction, arrhythmias and other heart related diseases. The present approach is based on immobilization of aptamers on the gate electrode which selectively binds to epinephrine with high affinity. The introduction of epinephrine in the system causes screening of negative charge of aptamers as well as the production of Faradaic current due to oxidation of epinephrine. The synergistic effect of these two events decreases the overall channel current which was seen in both transfer characteristics and current-time curve. Additional experiments against common interfering agents (dopamine, ascorbic acid, DOPAC etc) showed no decrease in the current which indicates high specificity of the sensor. Overall, the incorporation of aptamers in the transistor has allowed us to obtain a sensor exhibiting the lowest limit of detection for epinephrine (90 pM) till date which is comparable to normal physiological level. This approach provides a real-time detection of a large range of biomolecules and viral proteins in a time and cost-effective manner and has applications in point-of-care testing tool for several diagnostic applications.



中文翻译:

具有皮安级检测的基于高度适体的有机适体有机电化学生物传感器

已经开发了有机适体功能化的电化学晶体管来检测肾上腺素分子的存在,该肾上腺素分子充当兴奋性神经递质。肾上腺素水平的异常是某些疾病的直接症状,例如Takotsubo心肌病,心肌梗塞,心律不齐和其他与心脏有关的疾病。本方法基于将适体固定在栅电极上,其以高亲和力选择性结合肾上腺素。在系统中引入肾上腺素会导致筛选适体的负电荷,以及由于肾上腺素的氧化而产生法拉第电流。这两个事件的协同效应降低了总通道电流,这在传输特性和电流时间曲线中均可见。针对常见干扰剂(多巴胺,抗坏血酸,DOPAC等)的其他实验表明,电流没有降低,这表明传感器具有很高的特异性。总的来说,在晶体管中掺入适体使我们能够获得迄今为止显示出最低的肾上腺素检测限(90 pM)的传感器,可与正常生理水平相媲美。这种方法可以以时间和成本有效的方式实时检测各种生物分子和病毒蛋白,并在即时诊断工具中用于多种诊断应用。晶体管中适体的结合使我们获得了迄今为止显示出最低肾上腺素检测限(90 pM)的传感器,可与正常生理水平相媲美。这种方法可以以时间和成本有效的方式实时检测各种生物分子和病毒蛋白,并在即时诊断工具中用于多种诊断应用。晶体管中适体的结合使我们获得了迄今为止显示出最低肾上腺素检测限(90 pM)的传感器,可与正常生理水平相媲美。这种方法可以以时间和成本有效的方式实时检测各种生物分子和病毒蛋白,并在即时诊断工具中用于多种诊断应用。

更新日期:2018-05-22
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