当前位置: X-MOL 学术J. Food Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Speciation of Bio-Available Iodine in Abalone (Haliotis discus hannai ) by High-Performance Liquid Chromatography Hyphenated with Inductively Coupled Plasma-Mass Spectrometry Using an In Vitro Method
Journal of Food Science ( IF 3.9 ) Pub Date : 2018-05-22 , DOI: 10.1111/1750-3841.14173
Han Sol Doh 1 , Hyun Jin Park 1
Affiliation  

Abalone is one of the most valuable marine products found in East Asia because it is rich in nutritious substances including iodine. In this study, the in vitro dialyzability approach was used to assess the bio-available iodine species in abalone. Iodide, iodate, 3-iodo-L-tyrosine (MIT), and 3,5-diiodo-L-tyrosine (DIT) were separated by high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). To assure the consistency, reliability, and accuracy of the data, the method was validated. Comparison of the total iodine in abalone muscle and viscera indicated that abalone muscle showed greater digestion/absorption efficiency than abalone viscera (digestion efficiency: 68.13 ± 2.59% and 47.88 ± 5.76% and absorption efficiency: 59.78 ± 2.93% and 35.12 ± 1.43% for abalone viscera and muscle, respectively). However, evaluation of the sum of the analyzed iodine species targeted in this study by HPLC-ICP-MS indicated that abalone muscle showed lower digestion efficiency and similar absorption efficiency compared to that of abalone viscera (digestion efficiency: 35.52 ± 5.41% and 28.84 ± 1.83%; absorption efficiency: 23.56 ± 4.38% and 27.56 ± 1.51% for abalone viscera and muscle, respectively). The main forms of iodine detected in abalone muscle were iodide and MIT, whereas iodide was the major form in abalone viscera. PRACTICAL APPLICATION The bio-available iodine in abalone was quantified via an in vitro method employing HPLC-ICP-MS. The results of this study indicated that abalone is feasible as a new iodine source and may prospectively find application in iodine-fortified foods.

中文翻译:

高效液相色谱法与电感耦合等离子体质谱联用体外方法对鲍鱼 (Haliotis discus hannai ) 中的生物有效碘进行形态分析

鲍鱼是在东亚发现的最有价值的海产品之一,因为它富含包括碘在内的营养物质。在本研究中,体外可透析性方法用于评估鲍鱼中的生物可利用碘种类。碘化物、碘酸盐、3-碘-L-酪氨酸 (MIT) 和 3,5-二碘-L-酪氨酸 (DIT) 通过与电感耦合等离子体质谱联用的高效液相色谱法 (HPLC-ICP-MS) 分离)。为保证数据的一致性、可靠性和准确性,对该方法进行了验证。鲍鱼肌肉和内脏中总碘的比较表明,鲍鱼肌肉的消化/吸收效率高于鲍鱼内脏(消化效率:68.13 ± 2.59% 和 47.88 ± 5.76%,吸收效率:59.78 ± 2.93% 和 35.12 ± 1.43%鲍鱼的内脏和肌肉,分别)。然而,通过 HPLC-ICP-MS 对本研究中分析的碘种类总和的评估表明,与鲍鱼内脏相比,鲍鱼肌肉显示出较低的消化效率和相似的吸收效率(消化效率:35.52 ± 5.41% 和 28.84 ± 1.83%;吸收效率:鲍鱼内脏和肌肉的吸收效率分别为 23.56 ± 4.38% 和 27.56 ± 1.51%)。在鲍鱼肌肉中检测到的碘的主要形式是碘化物和MIT,而碘化物是鲍鱼内脏中的主要形式。实际应用 通过采用 HPLC-ICP-MS 的体外方法对鲍鱼中的生物有效碘进行定量。这项研究的结果表明,鲍鱼作为一种新的碘源是可行的,并可能在碘强化食品中得到应用。通过 HPLC-ICP-MS 对本研究中所分析的碘种类总和的评估表明,与鲍鱼内脏相比,鲍鱼肌肉的消化效率较低,吸收效率相似(消化效率:35.52 ± 5.41% 和 28.84 ± 1.83% ;吸收效率:鲍鱼内脏和肌肉的吸收效率分别为 23.56 ± 4.38% 和 27.56 ± 1.51%)。在鲍鱼肌肉中检测到的碘的主要形式是碘化物和MIT,而碘化物是鲍鱼内脏中的主要形式。实际应用 通过采用 HPLC-ICP-MS 的体外方法对鲍鱼中的生物有效碘进行定量。这项研究的结果表明,鲍鱼作为一种新的碘源是可行的,并可能在碘强化食品中得到应用。通过 HPLC-ICP-MS 对本研究中所分析的碘种类总和的评估表明,与鲍鱼内脏相比,鲍鱼肌肉的消化效率较低,吸收效率相似(消化效率:35.52 ± 5.41% 和 28.84 ± 1.83% ;吸收效率:鲍鱼内脏和肌肉的吸收效率分别为 23.56 ± 4.38% 和 27.56 ± 1.51%)。在鲍鱼肌肉中检测到的碘的主要形式是碘化物和MIT,而碘化物是鲍鱼内脏中的主要形式。实际应用 通过采用 HPLC-ICP-MS 的体外方法对鲍鱼中的生物有效碘进行定量。这项研究的结果表明,鲍鱼作为一种新的碘源是可行的,并可能在碘强化食品中得到应用。
更新日期:2018-05-22
down
wechat
bug