Tumor self-seeding occurs when circulating malignant cells reinfiltrate the original tumor. The process may breed more aggressive tumor cells, which may contribute to cancer progression. In this study, we observed tumor self-seeding in mouse xenograft models of hepatocellular carcinoma (HCC) for the first time. We confirmed that circulating tumor cell uptake of tumor-derived exosomes, which are increasingly recognized as key instigators of cancer progression by facilitating cell-cell communication, promoted tumor self-seeding by enhancing the invasive and migration capability of recipient HCC cells. Horizontal transfer of exosomal microRNA-25-5p to anoikis-resistant HCC cells significantly enhanced their migratory and invasive abilities, whereas inhibiting microRNA-25-5p alleviated these effects. Our experiments delineate an exosome-based novel pathway employed by functional microRNA from the original tumor cells that can influence the biological fate of circulating tumor cells.

中文翻译：

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肿瘤来源的外来体通过转移miRNA-25-5p来增强细胞运动性，从而促进肝细胞癌的肿瘤自发性。

当循环的恶性细胞重新浸润原始肿瘤时，就会发生肿瘤自我播种。该过程可能会繁殖出更具侵略性的肿瘤细胞，这可能有助于癌症的进展。在这项研究中，我们首次在肝细胞癌（HCC）小鼠异种移植模型中观察到了肿瘤自种。我们确认，越来越多地通过促进细胞与细胞之间的通讯，越来越多地认识到肿瘤来源的外泌体对肿瘤细胞的循环摄取，通过增强受体HCC细胞的侵袭和迁移能力促进了肿瘤的自我播种。将外泌体microRNA-25-5p水平转移至抗厌食症的HCC细胞可显着增强其迁移和侵袭能力，而抑制microRNA-25-5p可减轻这些影响。