Mitochondria are iron-rich organelles that are involved in the process of energy production through the electron-transporting system and heme synthesis. We developed a new mitochondria-targeted fluorescent probe, MtFluNox/Ac-MtFluNox, for Fe(II) based on N-oxide chemistry, which we recently established as a Fe(II)-selective fluorogenic switch. The deacetylated form MtFluNox showed a turn-on response towards Fe(II) with high metal selectivity in cuvette experiments, and an imaging study using its cell-compatible analogue Ac-MtFluNox demonstrated mitochondria-specific fluorescence enhancement in response to Fe(II) in living cells. Furthermore, the probe was able to detect endogenously accumulated Fe(II) induced as a result of the inhibition of heme synthesis.

中文翻译：

---

线粒体靶向荧光探针，用于选择性检测线粒体不稳定的Fe（ii）†

线粒体是富含铁的细胞器，通过电子传输系统和血红素合成参与能量产生过程。我们基于N氧化物化学为Fe（II）开发了一种新的针对线粒体的荧光探针MtFluNox / Ac-MtFluNox，我们最近将其建立为Fe（II）选择性荧光开关。脱乙酰化形式MtFluNox呈导通朝的Fe（响应II），在试管实验高的金属的选择性，并使用其细胞相容类似物的成像研究的Ac-MtFluNox表现出特定线粒体荧光增强响应为Fe（II）在活细胞中。此外，该探针能够检测由于抑制血红素合成而诱导的内源性积累的Fe（II）。